MOUSE ANTI HUMAN CD62E/CD62P | MyBioSource MBS213185 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN CD62E/CD62P

catalog :

MBS213185

quantity :

0.2 mg

price :

525 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1.2B6

reactivity :

human

application :

western blot, ELISA, immunoprecipitation, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS213185

products type :

Antibody

products full name :

MOUSE ANTI HUMAN CD62E/CD62P

products short name :

CD62E/CD62P

other names :

E-selectin; E-selectin; E-selectin; CD62 antigen-like family member E; ELAM-1; endothelial adhesion molecule 1; endothelial leukocyte adhesion molecule 1; leukocyte endothelial cell adhesion molecule 2; leukocyte-endothelial cell adhesion molecule 2; selectin E; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD_antigen: CD62E

products gene name :

CD62E/CD62P

other gene names :

SELE; SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2; ELAM1; ELAM-1; LECAM2

uniprot entry name :

LYAM2_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

1.2B6

host :

Mouse

reactivity :

Pig

sequence length :

610

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 1 mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)*

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Immunohistology - Frozen: Minimum Dilution: 1ug/ml; Maximum Dilution: 10ug/ml; Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections. . ELISA: Maximum Dilution: 10ug/ml. Flow Cytometry: Maximum Dilution: 10ug/ml. Western Blotting: Application Note: Clone 1.2B6 recognizes human CD62E/CD62P only under non-reducing conditions.

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

other info2 :

Immunogen: Human E-Selectin (ELAM-1). Histology Positive Control Tissue: Human Tonsil. Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Human

products description :

Mouse anti Human CD62E/CD62P antibody, clone 1.2B6 recognizes the human CD62E and CD62P cell surface antigens. Although previously thought to recognize only human CD62E, recent data (Goda et al. 2003) shows that Mouse anti Human CD62E/CD62P antibody, clone 1.2B6 also recognizes human CD62P, binding to a common epitope shared by these members of the selectin family.

ncbi gi num :

187960042

ncbi acc num :

NP_000441.2

ncbi gb acc num :

NM_000450.2

ncbi mol weight :

66,655 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Glucocorticoid Receptor Regulatory Network Pathway (138014); Hemostasis Pathway (106028); Malaria Pathway (152665); Malaria Pathway (152657)

ncbi summary :

The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008]

uniprot summary :

SELE: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. Belongs to the selectin/LECAM family. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q22-q25. Cellular Component: cortical cytoskeleton; extracellular space; perinuclear region of cytoplasm; plasma membrane; integral to membrane; coated pit; caveola; lipid raft. Molecular Function: protein binding; phospholipase binding; transmembrane receptor activity; sialic acid binding. Biological Process: heterophilic cell adhesion; leukocyte adhesion; phospholipase C activation; positive regulation of leukocyte migration; actin filament-based process; positive regulation of receptor internalization; regulation of inflammatory response; calcium-mediated signaling; response to lipopolysaccharide; inflammatory response; blood coagulation; leukocyte tethering or rolling; leukocyte migration; leukocyte migration during inflammatory response. Disease: Hypertension, Essential

size1 :

0.2 mg

price1 :

525 USD

more info or order :

MyBioSource product webpage