MOUSE ANTI HUMAN CD35 | MyBioSource MBS213087 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN CD35

catalog :

MBS213087

quantity :

0.1 mg

price :

230 USD

clonality :

monoclonal

host :

mouse

reactivity :

human

application :

western blot, FACS, flow cytometry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS213087

products type :

Antibody

products full name :

MOUSE ANTI HUMAN CD35

products short name :

CD35

other names :

complement receptor type 1 isoform F; Complement receptor type 1; complement receptor type 1; C3-binding protein; C3b/C4b receptor; CD35 antigen; Knops blood group antigen; complement component (3b/4b) receptor 1 (Knops blood group); C3b/C4b receptor; CD_antigen: CD35

products gene name :

CD35

other gene names :

CR1; CR1; KN; C3BR; C4BR; CD35; C3BR

uniprot entry name :

CR1_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

E11

host :

Mouse

sequence length :

2039

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 1 mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology Frozen, Flow cytometry (FC/FACS), Immunohistology Paraffin*, Western Blot (WB)

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Immunohistology - Frozen: Minimum Dilution: 1ug/ml; Maximum Dilution: 10ug/ml. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200. Immunohistology - Paraffin: Minimum Dilution: 1ug/ml; Maximum Dilution: 10ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

other info2 :

Immunogen: Human acute monocytic leukaemia cells. Histology Positive Control Tissue: Human Tonsil. Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Human

products description :

Mouse anti Human CD35 antibody, clone E11 recognizes CD35, a single chain cell surface glycoprotein which exists in four allotypic forms (A,B,C,D) of 190kD, 220kD, 160kD and 250kD respectively. The molecule is expressed by granulocytes, monocytes, B cells and some T cells. Functionally CD35 acts as a receptor for the C3b component of complement. Clone E11 has been reported to be suitable for use in Western Blotting (Birmingham et al. 1996)

ncbi gi num :

86793036

ncbi acc num :

NP_000564.2

ncbi gb acc num :

NM_000573.3

uniprot acc num :

P17927

ncbi mol weight :

223,663 Da

ncbi pathways :

Complement And Coagulation Cascades Pathway 198880!!Complement And Coagulation Cascades Pathway 83073!!Complement And Coagulation Cascades Pathway 484!!Complement Cascade Pathway 106405!!Hematopoietic Cell Lineage Pathway 83078!!Hematopoietic Cell Lineage Pathway 489!!Immune System Pathway 106386!!Innate Immune System Pathway 106387!!Legionellosis Pathway 469200!!Legionellosis Pathway 469186

ncbi summary :

This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized. [provided by RefSeq, Jul 2008]

size :

0.1 mg

price :

230 USD

more info or order :

MyBioSource product webpage