antibody

MOUSE ANTI HUMAN CD45RO

MBS213022

0.1 mg

230 USD

monoclonal

mouse

nonconjugated

UCHL1

immunoprecipitation, flow cytometry, FACS

Antibody

MOUSE ANTI HUMAN CD45RO

CD45RO

receptor-type tyrosine-protein phosphatase C isoform 1; Receptor-type tyrosine-protein phosphatase C; receptor-type tyrosine-protein phosphatase C; CD45 antigen; T200 glycoprotein; T200 leukocyte common antigen; protein tyrosine phosphatase, receptor type, c polypeptide; protein tyrosine phosphatase, receptor type, C; Leukocyte common antigen; L-CA; T200; CD_antigen: CD45

CD45RO

PTPRC; PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180; CD45; L-CA

PTPRC_HUMAN

Monoclonal

IgG2a

UCHL1

Mouse

1306

Purified. Purified IgG - liquid

IgG concentration 1 mg/ml

Store at 4 degree C or at -20 degree C if preferred. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistology Paraffin

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections.This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/50

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Human IL-2 dependent T cells. Histology Positive Control Tissue: Tonsil. Fusion Partners: Spleen cells from immunised mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Human

Mouse anti-human CD45RO monoclonal antibody, clone UCHL1 recognizes the low molecular weight isoform (180kD) of the leucocyte common antigen (LCA). The antigen is expressed by a functional subset of T cells with memory phenotype. In peripheral blood Mouse anti-human CD45RO monoclonal antibody, clone UCHL1 stains 40-80% of lymphocytes and all monocytes and granulocytes.

392307007

NP_002829.3

NM_002838.4

P08575

130,898 Da

Adaptive Immune System Pathway (366160); Axon Guidance Pathway (105688); B Cell Receptor Signaling Pathway (198909); BCR Signaling Pathway (138058); CXCR4-mediated Signaling Events Pathway (137910); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Developmental Biology Pathway (477129); EPO Receptor Signaling Pathway (198882); Fc Gamma R-mediated Phagocytosis Pathway (114228)

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]

CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described. Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor protein phosphatase, tyrosine; EC 3.1.3.48. Chromosomal Location of Human Ortholog: 1q31-q32. Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; external side of plasma membrane; lipid raft. Molecular Function: protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein kinase binding. Biological Process: B cell proliferation; axon guidance; substrate-bound cell migration, cell release from substrate; regulation of cell cycle; protein amino acid dephosphorylation; positive regulation of antigen receptor-mediated signaling pathway; T cell receptor signaling pathway; bone marrow development; B cell receptor signaling pathway; stem cell development; cell surface receptor linked signal transduction; dephosphorylation; positive regulation of protein kinase activity; release of sequestered calcium ion into cytosol; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; negative regulation of protein kinase activity; hemopoietic progenitor cell differentiation; positive regulation of T cell proliferation; negative regulation of cytokine and chemokine mediated signaling pathway; immunoglobulin biosynthetic process; defense response to virus; T cell differentiation. Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-positive; Hepatitis C Virus, Susceptibility To

0.1 mg

230 USD