MOUSE ANTI HUMAN F-ACTIN | MyBioSource MBS212994 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN F-ACTIN

catalog :

MBS212994

quantity :

0.5 mg

price :

410 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

NH3

reactivity :

human

application :

western blot, ELISA, flow cytometry, FACS, enzyme immunoassay

product information

catalog number :

MBS212994

products type :

Antibody

products full name :

MOUSE ANTI HUMAN F-ACTIN

products short name :

ACTIN F TYPE

other names :

actin, cytoplasmic 2; Actin, cytoplasmic 2; actin, cytoplasmic 2; cytoskeletal gamma-actin; epididymis luminal protein 176; actin gamma 1; Gamma-actinActin, cytoplasmic 2, N-terminally processed

other gene names :

ACTG1; ACTG1; ACT; ACTG; BRWS2; DFNA20; DFNA26; HEL-176; ACTG

uniprot entry name :

ACTG_HUMAN

clonality :

Monoclonal

isotype :

IgM

clone :

NH3

host :

Mouse

sequence length :

375

form :

Purified. Purified IgM - liquid

concentration :

IgM concentration 1.0 mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS), Western Blot (WB)

app notes :

ELISA: Maximum Dilution: 1 / 10;. Flow Cytometry: Maximum Dilution: 1 / 10;. Western Blotting: Minimum Dilution: 1/100; Maximum Dilution: 1/500

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgM prepared from tissue culture supernatant

other info2 :

Immunogen: Human monocytes and U937 cell line. Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Human

products description :

Mouse anti Human F-Actin antibody, clone NH3 recognizes human Filamentous actin (F-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types.

ncbi gi num :

316659409

ncbi acc num :

NP_001186883.1

ncbi gb acc num :

NM_001199954.1

ncbi mol weight :

41,793 Da

ncbi pathways :

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Axon Guidance Pathway (105688); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Developmental Biology Pathway (477129); Dilated Cardiomyopathy Pathway (121494)

ncbi summary :

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011]

uniprot summary :

ACTG1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: 17q25. Cellular Component: filamentous actin; extracellular space; cytoskeleton; myofibril; membrane; plasma membrane; nucleus; cytosol. Molecular Function: identical protein binding; protein binding; structural constituent of cytoskeleton; ubiquitin protein ligase binding; ATP binding. Biological Process: retinal homeostasis; intercellular junction assembly and maintenance; axon guidance; ephrin receptor signaling pathway; innate immune response; sarcomere organization; cell motility; vascular endothelial growth factor receptor signaling pathway. Disease: Baraitser-winter Syndrome 2; Deafness, Autosomal Dominant 20

size1 :

0.5 mg

price1 :

410 USD