RAT ANTI HUMAN CD8 | MyBioSource MBS212993 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RAT ANTI HUMAN CD8

catalog :

MBS212993

quantity :

0.2 mg

price :

430 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

[YTC182.20]

reactivity :

human, cow, Rhesus monkey

application :

flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Staining of human peripheral blood lymphocytes with Rat anti Human CD8

image 2 :

Staining of human peripheral blood lymphocytes with Rat anti Human CD8:FITC (MBS211011)

product information

catalog number :

MBS212993

products type :

Antibody

products full name :

RAT ANTI HUMAN CD8

products short name :

[CD8]

products name syn :

[CD8B1, MAL]

other names :

[T-cell surface glycoprotein CD8 alpha chain isoform 1; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; CD8 antigen, alpha polypeptide (p32); Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T-cell antigen Leu2; T-lymphocyte differentiation antigen T8/Leu-2; CD8a molecule; T-lymphocyte differentiation antigen T8/Leu-2; CD_antigen: CD8a]

products gene name :

[CD8]

other gene names :

[CD8A; CD8A; CD8; MAL; p32; Leu2; MAL]

uniprot entry name :

CD8A_HUMAN

clonality :

Monoclonal

isotype :

IgG2b

clone :

[YTC182.20]

host :

Rat

reactivity :

Reacts with: Cynomolgus monkey, Rhesus Monkey. N.B. Antibody reactivity and working conditions may vary between species.

sequence length :

235

form :

Purified. Purified IgG - liquid

concentration :

Ig concentration 1.0mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch.

tested application :

Immunohistology Frozen, Flow cytometry (FC/FACS)

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200

image1 heading :

Testing Data

image2 heading :

Testing Data

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

other info2 :

Immunogen: Mouse L cells transfected with human CD8 gene. Fusion Partners: Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag.1.2.3 myeloma line. Buffer Solution: Phosphate buffered saline. Target Species: Human

products description :

Rat anti Human CD8 antibody, clone YTC182.20 recognizes the human CD8 cell surface antigen, a 30/32kD glycoprotein expressed by the cytotoxic/suppressor subset of T lymphocytes, and more weakly by NK cells. Rat anti Human CD8 antibody, clone YTC182.20 recognizes a different epitope from clone YTC141.1.

ncbi gi num :

225007536

ncbi acc num :

NP_001139345.1

ncbi gb acc num :

NM_001145873.1

ncbi mol weight :

30,214 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); IL12-mediated Signaling Events Pathway (137922); Immune System Pathway (106386)

ncbi summary :

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

uniprot summary :

CD8A: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 2p12. Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; extracellular region; external side of plasma membrane. Molecular Function: protein binding; protein homodimerization activity; MHC class I protein binding; coreceptor activity; protein kinase binding. Biological Process: antigen processing and presentation; regulation of immune response; T cell activation; T cell mediated immunity; cytotoxic T cell differentiation; positive regulation of calcium-mediated signaling; immune response; defense response to virus; transmembrane receptor protein tyrosine kinase signaling pathway. Disease: Cd8 Deficiency, Familial

size1 :

0.2 mg

price1 :

430 USD

more info or order :

MyBioSource product webpage