antibody

MOUSE ANTI RAT CD45RA (B CELLS ONLY)

MBS212980

0.1 mg

185 USD

monoclonal

mouse

nonconjugated

OX-33

flow cytometry, FACS

Antibody

MOUSE ANTI RAT CD45RA (B CELLS ONLY)

CD45RA

receptor-type tyrosine-protein phosphatase C isoform 3; Receptor-type tyrosine-protein phosphatase C; receptor-type tyrosine-protein phosphatase C; Protein tyrosine phosphatase, receptor-type, c polypeptide; T200 glycoprotein; leucocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; protein tyrosine phosphatase, receptor type, C; Leukocyte common antigen; L-CA; T200; CD_antigen: CD45

CD45RA

Ptprc; Ptprc; Lca; RT7; CD45; L-CA; T200; L-CA

PTPRC_RAT

Monoclonal

IgG1

OX-33

Mouse

1190

Purified. Purified IgG - liquid

IgG concentration 1.0 mg/ml

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen, Flow cytometry (FC/FACS), Immunohistology Paraffin*

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100. Immunohistology - Paraffin: Application Note: PLP fixation is recommended for optimal results, see Whiteland et al. for details

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Purified rat spleen leucocyte common antigen. Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the NS0/U myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Rat

Mouse anti Rat CD45RA antibody, clone OX-33 is directed against a high molecular weight band of the leucocyte common antigen. MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labelling in bone marrow and none on thymocytes (Barclay et al. 1987).

158138490

NP_001103357.1

NM_001109887.1

P04157

134,182 Da

B Cell Receptor Signaling Pathway (198486); Cell Adhesion Molecules (CAMs) Pathway (83461); Cell Adhesion Molecules (CAMs) Pathway (480); EPO Receptor Signaling Pathway (198514); Fc Gamma R-mediated Phagocytosis Pathway (114013); Fc Gamma R-mediated Phagocytosis Pathway (106590); Primary Immunodeficiency Pathway (83513); Primary Immunodeficiency Pathway (537); T Cell Receptor Signaling Pathway (198537); T Cell Receptor Signaling Pathway (83472)

member of a family of heavily glycosylated leukocyte cell surface glycoproteins; displays extensive O-glycosylation [RGD, Feb 2006]

CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described. Protein type: Motility/polarity/chemotaxis; Receptor protein phosphatase, tyrosine; Membrane protein, integral; EC 3.1.3.48. Cellular Component: internal side of plasma membrane; cell surface; focal adhesion; membrane; integral to plasma membrane; plasma membrane; integral to membrane; intracellular; cytosol; lipid raft; external side of plasma membrane. Molecular Function: heparan sulfate proteoglycan binding; heparin binding; protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein kinase regulator activity; protein kinase binding. Biological Process: B cell proliferation; positive regulation of isotype switching to IgG isotypes; regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of T cell mediated cytotoxicity; regulation of cell cycle; activation of MAPK activity; protein amino acid dephosphorylation; T cell receptor signaling pathway; T cell proliferation; leukocyte adhesion; B cell receptor signaling pathway; positive regulation of MAPKKK cascade; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; response to gamma radiation; positive regulation of T cell mediated immunity; positive regulation of T cell proliferation; negative regulation of protein amino acid autophosphorylation; negative regulation of cytokine and chemokine mediated signaling pathway; positive regulation of gamma-delta T cell differentiation; defense response to virus; positive thymic T cell selection; T cell differentiation; regulation of B cell receptor signaling pathway; positive regulation of humoral immune response mediated by circulating immunoglobulin; negative regulation of peptidyl-tyrosine phosphorylation; substrate-bound cell migration, cell release from substrate; positive regulation of antigen receptor-mediated signaling pathway; negative thymic T cell selection; bone marrow development; heterotypic cell-cell adhesion; stem cell development; positive regulation of T cell differentiation; dephosphorylation; B cell differentiation; release of sequestered calcium ion into cytosol; regulation of B cell differentiation; negative regulation of protein kinase activity; hemopoietic progenitor cell differentiation; positive regulation of alpha-beta T cell proliferation; immunoglobulin biosynthetic process

0.1 mg

185 USD