MOUSE ANTI ALIX | MyBioSource MBS212843 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI ALIX

catalog :

MBS212843

quantity :

0.1 mg

price :

390 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3A9

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, enzyme immunoassay

product information

catalog number :

MBS212843

products type :

Antibody

products full name :

MOUSE ANTI ALIX

products short name :

ALIX

other names :

programmed cell death 6-interacting protein isoform 2; Programmed cell death 6-interacting protein; programmed cell death 6-interacting protein; ALG-2 interacting protein 1; ALG-2-interacting protein X; PDCD6-interacting protein; apoptosis-linked gene 2-interacting protein X; dopamine receptor interacting protein 4; programmed cell death 6 interacting protein; ALG-2-interacting protein 1; ALG-2-interacting protein X; Hp95

products gene name :

ALIX

other gene names :

PDCD6IP; PDCD6IP; AIP1; ALIX; HP95; DRIP4; AIP1; ALIX; KIAA1375; PDCD6-interacting protein

uniprot entry name :

PDC6I_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

3A9

host :

Mouse

reactivity :

Human, Mouse, Rat, Xenopus

sequence length :

271

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 1.0mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin, Western Blot (WB)

app notes :

Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections.

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide (NaN3) . Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

other info2 :

Buffer Solution: Phosphate buffered saline

products description :

Mouse anti ALIX antibody, clone 3A9 recognizes the apoptosis-linked gene-2 interacting protein X (ALIX), also known as Programmed cell death 6-interacting protein or Hp95. ALIX is a 868 amino acid ~90kDa cytoplasmic protein containing a single BRO1 domain implicated in endosomal targeting (Kim et al. 2005). ALIX was first reported to play a role in apoptosis dependent on its association with PDCD6 (Mahul-Mellier et al. 2006) but has subsequently shown to be involved in endocytosis (Matsuo et al. 2004), viral budding (Irie et al. 2008) and actin cytoskeletal assembly (Pan et al. 2006). Mouse anti Alix, clone 3A9 has been used extensively for the detection of ALIX by Western blotting and for the identification of an ALIX homologue in the sea urchin Paracentrotus lividus (Romancino et al. 2013).

ncbi gi num :

241982780

ncbi acc num :

NP_001155901.1

ncbi gb acc num :

NM_001162429.2

uniprot acc num :

Q8WUM4

ncbi mol weight :

30,453 Da

ncbi pathways :

Budding And Maturation Of HIV Virion Pathway (106009); Disease Pathway (530764); Endocytosis Pathway (102279); Endocytosis Pathway (102181); HIV Infection Pathway (105983); HIV Life Cycle Pathway (105984); Late Phase Of HIV Life Cycle Pathway (105995); Uptake And Actions Of Bacterial Toxins Pathway (1127565); Uptake And Function Of Anthrax Toxins Pathway (1127574)

ncbi summary :

This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]

uniprot summary :

Alix: an adaptor protein that may regulate the function of receptor and cytoskeleton-associated tyrosine kinases. A class E vacuolar protein sorting (VPS) factor involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. In case of infection, the HIV-1 virus directly takes advantage of its function for viral exocytosis and budding, via its interaction with HIV-1 p6 protein. May play a role in the regulation of both apoptosis and cell proliferation. Protein type: Apoptosis; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 3p22.3. Cellular Component: focal adhesion; membrane; cytoplasm; melanosome; microtubule organizing center; immunological synapse; cytosol; vesicle. Molecular Function: protein binding; protein homodimerization activity; proteinase activated receptor binding; SH3 domain binding; calcium-dependent protein binding. Biological Process: protein transport; cell separation during cytokinesis; viral reproduction; apoptosis; viral infectious cycle; mitotic metaphase plate congression; nuclear organization and biogenesis

size1 :

0.1 mg

price1 :

390 USD