antibody

MOUSE ANTI DUCK IgY HEAVY CHAIN

MBS212832

0.25 mg

450 USD

monoclonal

mouse

nonconjugated

[16C7]

western blot, ELISA, flow cytometry, FACS, enzyme immunoassay

Antibody

MOUSE ANTI DUCK IgY HEAVY CHAIN

[IgY]

[IgY]

Monoclonal

IgG1

[16C7]

Mouse

Goose. Does not react with Chicken. N.B. Antibody reactivity and working conditions may vary between species.

IgY Heavy Chain

Purified IgG - liquid

IgG concentration 0.5mg/ml

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

ELISA (EIA), Flow cytometry (FC/FACS), Western Blot (WB)

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10 6 cells in 100ul. Western Blotting: Maximum Dilution: non-reducing

Perservative Stabilisers: 0.09% Sodium Azide (NaN3)

Immunogen: Purified Pekin duck yolk IgY. Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Buffer Solution: Phosphate buffered saline. Target Species: Duck. Specificity Note: MBS212832 recognizes both the IgY and IgY(-Fc) Pekin duck heavy chain molecules. The duck IgY molecule, consisting of two heavy (H) and two light (L) chains, has been categorized as the 7.8S full-length IgY form and the 5.7S truncated IgY(-Fc) form. Studies have shown that the IgY(-Fc) molecule possesses the H chain Cu1 and Cu2 constant domains, but is lacking the Cu3 and Cu4 constant region terminal domains, all four of which are present in the full-length IgY molecule. Thus the structure of the IgY(-Fc) molecule resembles that of a F(ab’)2 fragment of IgY. MBS212832 also detects IgY heavy chain in Mallard ducks, goose and swan. MBS212832 detects bands of approximately 118kDa and 178-200kDa in Pekin duck cell lysates under non-reducing conditions in Western blotting.

MBS212832 recognises both the IgY and IgY(-Fc) Pekin duck heavy chain molecules. The duck IgY molecule, consisting of two heavy (H) and two light (L) chains, has been categorized as the 7.8S full-length IgY form and the 5.7S truncated IgY(-Fc) form. Studies have shown that the IgY(-Fc) molecule possesses the H chain Cu1 and Cu2 constant domains, but is lacking the Cu3 and Cu4 constant region terminal domains, all four of which are present in the full-length IgY molecule. Thus the structure of the IgY(-Fc) molecule resembles that of a F(ab')2 fragment of IgY. MBS212832 also detects IgY heavy chain in Mallard ducks, goose and swan. MBS212832 detects bands of approximately 118kDa and 178-200kDa in Pekin duck cell lysates under non-reducing conditions in Western blotting.

0.25 mg

450 USD