catalog number :
MBS212828
products full name :
MOUSE ANTI HUMAN IgG (Fc) (CH2 DOMAIN)
products short name :
IgG
other names :
Ig gamma-3 chain C region; Ig gamma-3 chain C region; immunoglobulin heavy constant gamma 3 (G3m marker); HDC; Heavy chain disease protein
other gene names :
IGHG3; IGHG3; IgG3
uniprot entry name :
IGHG3_HUMAN
form :
Purified. Purified IgG - liquid
concentration :
IgG concentration 1.0 mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS)
app notes :
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
other info2 :
Immunogen: Human IgG polyclonal. Fusion Partners: Spleen cells from immunised Balb/c mouse were fused with cells of the P3-NS1/Ag1 myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Human
products description :
Mouse anti Human IgG (Fc) (CH2 Domain) antibody, clone 8A4 recognizes an epitope within the CH2 domain of the Fc region of all subclasses of human IgG.
ncbi mol weight :
41,287 Da
ncbi pathways :
Classical Antibody-mediated Complement Activation Pathway (106409); Complement Cascade Pathway (106405); Creation Of C4 And C2 Activators Pathway (106407); FCGR Activation Pathway (771578); Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway (771577); IL4-mediated Signaling Events Pathway (137933); Immune System Pathway (106386); Initial Triggering Of Complement Pathway (106406); Innate Immune System Pathway (106387); Regulation Of Actin Dynamics For Phagocytic Cup Formation Pathway (771579)
uniprot summary :
IGHG3: Dimer linked by 12 disulfide bonds; it has an extra interchain disulfide bond at position 7 in addition to the 11 normally present in the hinge region. Chromosomal Location of Human Ortholog: 14q32.33. Cellular Component: extracellular space; extracellular region. Molecular Function: antigen binding. Biological Process: retinal homeostasis; innate immune response; complement activation, classical pathway; complement activation