MOUSE ANTI HUMAN CD19 | MyBioSource MBS212806 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN CD19

catalog :

MBS212806

quantity :

0.2 mg

price :

610 USD

clonality :

monoclonal

host :

mouse

reactivity :

human

application :

western blot, enzyme immunoassay, ELISA, FACS, flow cytometry, immunoprecipitation

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS212806

products type :

Antibody

products full name :

MOUSE ANTI HUMAN CD19

products short name :

CD19

other names :

B-lymphocyte antigen CD19 isoform 1; B-lymphocyte antigen CD19; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; T-cell surface antigen Leu-12; differentiation antigen CD19; CD19 molecule; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD_antigen: CD19

products gene name :

CD19

other gene names :

CD19; CD19; B4; CVID3; N/A

uniprot entry name :

CD19_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

LE-CD19

host :

Mouse

sequence length :

557

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 1.0 mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

ELISA (EIA), Flow cytometry (FC/FACS)*, Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. . Immunohistology - Paraffin: Minimum Dilution: 1/100; Maximum Dilution: 1/200; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. . Western Blotting: Maximum Dilution: Reducing conditions

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

other info2 :

Histology Positive Control Tissue: Tonsil. Buffer Solution: Phosphate buffered saline. Target Species: Human

products description :

Mouse anti Human CD19 antibody, clone LE-CD19 recognizes an epitope within the C-terminal cytoplasmic tail sequence of human CD19, a single pass type I transmembrane glycoprotein containing two C2 type Ig-like domains in the N-terminal extracellular region and four potential phosphorylation sites for tyrosine together with a single serine in the cytoplasmic region. Human CD19 is expressed on virtually all cells of the B-cell lineage with the exception of plasma cells and plays a regulatory role in B-cell differentiation and prloliferation. B-cells are essential for antibody production and mutations in the CD19 gene can lead to an immunodeficiency syndrome, CIVD3 characterized by hypogammaglobulinemia leading to recurrent infections and the inability to mount an antibody mediated response to immune insult. Although immunoglobulin production is impaired B-cell precursors appear in normal numbers together with some reduction in more mature B-cell forms (van Zelm et al. 2006). B-cells have also been implicated in the progression and pathogenesis of multiple sclerosis and are common components of both active and chronic MS lesions and well as the CSF (Ritchie et al. 2004) Mouse anti Human CD19 antibody, clone LE-CD19 has been successfully employed for the immunohistochemical demonstration of CD19 in formalin fixed, paraffin embedded tissues (Streeck, H. et al. 2011) and for the detection of CD19 in cell lysates by Western blotting.

ncbi gi num :

296010921

ncbi acc num :

NP_001171569.1

ncbi gb acc num :

NM_001178098.1

uniprot acc num :

P15391

ncbi mol weight :

61,200 Da

ncbi pathways :

Adaptive Immune System Pathway 366160!!Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway 576249!!B Cell Receptor Signaling Pathway 198909!!B Cell Receptor Signaling Pathway 83081!!B Cell Receptor Signaling Pathway 492!!BCR Signaling Pathway 138058!!Constitutive PI3K/AKT Signaling In Cancer Pathway 685535!!DAP12 Interactions Pathway 685549!!DAP12 Signaling Pathway 685550!!Disease Pathway 530764

ncbi summary :

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]

size :

0.2 mg

price :

610 USD

more info or order :

MyBioSource product webpage