antibody

MOUSE ANTI HUMAN SOCS-3

MBS212753

0.1 mg

315 USD

monoclonal

mouse

nonconjugated

SO1

western blot, ELISA, enzyme immunoassay

Antibody

MOUSE ANTI HUMAN SOCS-3

SOCS-3

suppressor of cytokine signaling 3; Suppressor of cytokine signaling 3; suppressor of cytokine signaling 3; STAT-induced STAT inhibitor 3; cytokine-inducible SH2 protein 3; suppressor of cytokine signaling 3; Cytokine-inducible SH2 protein 3; CIS-3; STAT-induced STAT inhibitor 3; SSI-3

SOCS-3

SOCS3; SOCS3; CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3; CIS3; SSI3; SOCS-3; CIS-3; SSI-3

SOCS3_HUMAN

Monoclonal

IgG2b

SO1

Mouse

225

Purified. Purified IgG - liquid

Ig concentration 1.0 mg/ml

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Shelf Life: 18 months from date of despatch.

ELISA (EIA), Western Blot (WB)

Western Blot: This item detects a band of approximately 30kDa in Hela cell lysates. Western Blotting: Minimum Dilution: 1/100; Maximum Dilution: 1/500

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution: Phosphate buffered saline. Target Species: Human

Mouse anti Human SOCS-3 antibody, clone SO1 recognizes the human suppressor of cytokine signaling 3 protein, also known as SOCS-3, cytokine-inducible SH2 protein 3 or STAT-induced STAT inhibitor 3. SOCS-3 is a 225 amino acid, 30kDa member of the suppressor of cytokine signaling family (Starr et al. 1997) containing a single SH2 and a single SOCS box domain. SOCS-3 acts as a negative regulator of Janus Kinase (JAK) and signal transducer and activators of transcription (STAT) signaling pathways. SOCS-3 specifically acts as a negative feedback regulator for the STAT3 pathways, by directly binding to the Tyr-759 motif of the IL-6 signal-transducing receptor subunit gp130, thereby preventing the IL-6 activation of STAT3. The expression of SOCS-3 is inducible by numerous factors, including IL-6, IL-10, Toll-like receptor (TLR) agonists and IFN gamma, making this protein the focus of research into inflammatory diseases, such as Arthritis, Colitis, and Crohn's Disease (Elliott and Johnstone 2004). SOCS-3 expression has also been shown to affect IFN alpha sensitivity in tumour studies (Brender et al.2001). Notch signaling plays a critical role in the determination of M1 versus M2 macrophage polarization, and that RBPJ -mediated Notch signaling, regulates this polarization through SOCS-3 (Narayana and Balaji 2008, Schroeder and Just 2000).

45439352

NP_003946.3

NM_003955.4

O14543

24,770 Da

ATF-2 Transcription Factor Network Pathway (138006); Adaptive Immune System Pathway (366160); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Cytokine Signaling In Immune System Pathway (366171); ECS Complex Pathway (413455); ECS Complex Pathway (468394)

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. [provided by RefSeq, Jul 2008]

SOCS3: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo. Probable substrate recognition component of a SCF-like ECS (Elongin BC- CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST. Interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. Binding to JAK2 is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK2 JH1 domain. Binds specific activated tyrosine residues of the leptin, EPO, IL12, GSCF and gp130 receptors. Interaction with CSNK1E stabilizes SOCS3 protein. Component of the probable ECS(SOCS3) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SOCS3. Interacts with CUL5, RNF7, TCEB1 and TCEB2. Interacts with CUL2. Interacts with FGFR3. Interacts with INSR. Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus. Protein type: Inhibitor. Chromosomal Location of Human Ortholog: 17q25.3. Cellular Component: cytoplasm; cytosol. Molecular Function: protein binding; protein kinase inhibitor activity. Biological Process: negative regulation of inflammatory response; regulation of growth; cytokine and chemokine mediated signaling pathway; negative regulation of protein kinase activity; protein ubiquitination; negative regulation of insulin receptor signaling pathway; positive regulation of cell differentiation; JAK-STAT cascade; negative regulation of JAK-STAT cascade; negative regulation of apoptosis. Disease: Dermatitis, Atopic, 4

0.1 mg

315 USD