antibody

MOUSE ANTI BOVINE INTERLEUKIN-4

MBS212744

0.5 mg

545 USD

monoclonal

mouse

nonconjugated

[CC313]

ELISA, enzyme immunoassay

Antibody

MOUSE ANTI BOVINE INTERLEUKIN-4

[IL-4]

[interleukin-4; Interleukin-4; interleukin-4; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; interleukin 4; B-cell stimulatory factor 1; BSF-1; Lymphocyte stimulatory factor 1]

[IL4; IL4; IL-4; BSF-1; IL-4; BSF-1]

IL4_BOVIN

Monoclonal

IgG2a

[CC313]

Mouse

Goat, Sheep. N.B. Antibody reactivity and working conditions may vary between species.

IL-4

Purified IgG - liquid, Phosphate buffered saline

IgG concentration 1.0 mg/ml

Store at 4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

ELISA (EIA)

ELISA: 2ug/ml-5ug/ml. Flow Cytometry: Use 10ul of the suggested working dilution to label 10 6 cells in 100ul.

Preservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant. Immunogen: Recombinant bovine IL-4. Carrier Free: Yes

Target Species: Bovine. Fusion Partners: Spleen cells form imunized BALB/c mice were fused with cells of the SP2/O myeloma cell line.

Mouse anti Bovine interleukin-4, clone CC313 is specific for bovine interleukin 4 (IL-4), also known as B-cell stimulatory factor 1. Bovine interleukin-4 is a 15kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6kDa active cytokine, the apparent Mw may be altered by glycosylation (Heussler et al. 1992). IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses. Mouse anti Bovine interleukin-4, clone CC313 has been used successfully as a coating antibody in a sandwich ELISA with Clone CC314 as a detection reagent both with bovine (Stabel et al. 2011) and ovine samples (Olivier et al. 2012). Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples (Marinaro et al. 2012).

27806871

NP_776346.1

NM_173921.2

P30367

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages.

0.5 mg

545 USD