antibody

MOUSE ANTI HUMAN p21/WAF1

MBS212705

0.1 mg

320 USD

monoclonal

mouse

nonconjugated

WA-1

western blot, immunoprecipitation

Antibody

MOUSE ANTI HUMAN p21/WAF1

p21/WAF1

cyclin-dependent kinase inhibitor 1 isoform 1; Cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; CDK-interaction protein 1; DNA synthesis inhibitor; melanoma differentiation associated protein 6; wild-type p53-activated fragment 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21

p21/WAF1

CDKN1A; CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1; CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; MDA-6

CDN1A_HUMAN

Monoclonal

IgG1

WA-1

Mouse

164

This item recognises the human cyclin-dependent kinase inhibitor p21/WAF1, which is an important cell cycle regulatory protein, also known as CIP1.The p21 protein inhibits cell cycle progression at both the G1 and G2 checkpoints. The expression of p21 is inducible by a wide range of stress stimuli.This item detects a band of approximately 21kD in MCF-7 cell lysates following UV radiation.

Purified. Purified IgG - liquid

IgG concentration 1.0 mg/ml

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunoprecipitation (IP), Western Blot (WB)

Western Blotting: Minimum Dilution: 1/100; Maximum Dilution: 1/1000

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Ig fraction prepared by ammonium sulphate precipitation

Immunogen: Human p21 protein. Buffer Solution: Phosphate buffered saline. Target Species: Human

MBS212705 recognises the human cyclin-dependent kinase inhibitor p21/WAF1, which is an important cell cycle regulatory protein, also known as CIP1. The p21 protein inhibits cell cycle progression at both the G1 and G2 checkpoints. The expression of p21 is inducible by a wide range of stress stimuli. MBS212705 detects a band of approximately 21kD in MCF-7 cell lysates following UV radiation.

11386203

NP_000380.1

NM_000389.4

P38936

18,119 Da

AKT Phosphorylates Targets In The Cytosol Pathway (106475); AMPK Signaling Pathway (198868); Adaptive Immune System Pathway (366160); Adipogenesis Pathway (198832); AhR Pathway (755436); Alpha6-Beta4 Integrin Signaling Pathway (198807); Androgen Receptor Signaling Pathway (198806); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527)

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Nov 2010]

p21Cip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. Its expression is tightly controlled by p53, through which this protein mediates the p53-dependent cell cycle arrest at G1 phase. Protein type: Cell cycle regulation; Inhibitor. Chromosomal Location of Human Ortholog: 6p21.2. Cellular Component: nucleoplasm; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cyclin-dependent protein kinase holoenzyme complex; cytosol; nucleus. Molecular Function: cyclin-dependent protein kinase inhibitor activity; cyclin binding; protein binding; cyclin-dependent protein kinase activating kinase activity; metal ion binding; ubiquitin protein ligase binding; protein complex binding. Biological Process: nerve growth factor receptor signaling pathway; response to toxin; response to arsenic; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; protein amino acid phosphorylation; negative regulation of cell proliferation; positive regulation of fibroblast proliferation; positive regulation of B cell proliferation; G2/M transition of mitotic cell cycle; cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to X-ray; response to corticosterone stimulus; epidermal growth factor receptor signaling pathway; response to drug; organ regeneration; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; negative regulation of cyclin-dependent protein kinase activity; response to organic nitrogen; positive regulation of programmed cell death; response to hyperoxia; positive regulation of protein kinase activity; Ras protein signal transduction; cellular response to extracellular stimulus; innate immune response; regulation of protein import into nucleus, translocation; negative regulation of phosphorylation; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; negative regulation of cell growth; response to DNA damage stimulus; G1/S transition of mitotic cell cycle; negative regulation of apoptosis

0.1 mg

320 USD