MyBioSource

antibody

MOUSE ANTI FELINE IMMUNODEFICIENCY VIRUS p24 gag

MBS212642

0.25 mg

485 USD

monoclonal

mouse

enzyme immunoassay, western blot, immunocytochemistry, FACS, ELISA, flow cytometry

MBS212642

Antibody

MOUSE ANTI FELINE IMMUNODEFICIENCY VIRUS p24 gag

FELINE IMMUNODEFICIENCY VIRUS p24 gag

Monoclonal

IgG1

PAK3-2C1

Mouse

Purified. Purified IgG - liquid

IgG concentration 1.0 mg/ml

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Western Blot (WB)

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections. . Flow Cytometry: Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.

Perservative Stabilisers: 0.05% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution: Phosphate buffered saline. Target Species: Viral

Mouse anti Feline Immunodeficiency Virus p24 gag antibody, clone PAK3-2C1 recognizes an immunodominant p24 epitope of feline immunodeficiency virus (FIV) p24 gag. The lentivirus FIV, responsible for a progressive and debilitating immune deficiency syndrome in domestic cats, similar to that caused by the human Immunodeficiency Virus (HIV), is a complex retrovirus with a tightly-packed genome, containing the structural genes gag, env and pro-pol and the accessory genes vif, rev and ORF-A/2. The specific binding epitope recognized by Mouse anti Feline Immunodeficiency Virus p24 gag antibody, clone PAK3-2C1 appears to be enclosed in a tight peptide coil during gag production and maturation. The addition of 0.3% Triton-X detergent is recommended to reveal this epitope for antibody binding. Mouse anti Feline Immunodeficiency Virus p24 gag antibody, clone PAK3-2C1 detects a dominant band of approximately 24kDa, and also detects p24 gag precursor bands at 36, 39, 49 and 52kDa under reducing conditions in Western blotting. Mouse anti Feline Immunodeficiency Virus p24 gag antibody, clone PAK3-2C1 does not recognize Feline Leukaemia Virus (FeLV), Feline Herpes Virus (FHV) type 1, Feline Coronavirus or Feline Calicivirus.

0.25 mg

485 USD