antibody

MOUSE ANTI 5-METHYLCYTIDINE

MBS212567

0.1 mg

550 USD

monoclonal

mouse

nonconjugated

33D3

western blot, immunocytochemistry, flow cytometry, FACS

Antibody

MOUSE ANTI 5-METHYLCYTIDINE

5-METHYLCYTIDINE

Monoclonal

IgG1

33D3

Mouse

Bovine, Human, Mouse, Rat

Purified. Purified

IgG concentration 1.0 mg/ml

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunoblotting (IB), Immunofluorescence (IF), Methylated DNA Immunoprecipitation (MP), Immunohistology Paraffin*

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Flow Cytometry: Application Note: Membrane permeabilization may be required prior to staining. Cell pretreatment before staining is described in Giraldo et al (2007) . . Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Perservative Stabilisers: 0.01% Thiomersal

Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0-Ag 14 myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Broad

Mouse anti 5-MeCyd antibody, clone 33D3 recognizes 5-MethylCytidine, a modified base found in the DNA of plants and vertebrates. Methylation of DNA is an epigenetic process that stably alters the expression of genes in cells as they divide and differentiate into specific tissues. The resulting change is normally permanent and unidirectional, preventing a cell from reverting to a stem cell or converting into another type of tissue. In cancer biology, DNA hypermethylation is associated with gene silencing while hypomethylation is reported to be associated with disease progression (Sincic & Herceg, 2011). Mouse anti 5-MeCyd antibody, clone 33D3 is specific to the methylated base and has minimal reactivity to non-methylated cytidine or cytosine (Reynaudet al.1992) Mouse anti 5-MeCyd antibody, clone 33D3 has been reported for use in Methylated DNA immunoprecipitation (MeDIP) (Ponteset al. 2007).

0.1 mg

550 USD