RAT ANTI CCT EPSILON | MyBioSource MBS212546 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RAT ANTI CCT EPSILON

catalog :

MBS212546

quantity :

0.1 mg

price :

485 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

PK/29/23/8d

reactivity :

human, mouse, rat, cow

application :

western blot, immunoprecipitation, EMSA

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS212546

products type :

Antibody

products full name :

RAT ANTI CCT EPSILON

products short name :

CCT EPSILON

other names :

T-complex protein 1 subunit epsilon; T-complex protein 1 subunit epsilon; T-complex protein 1 subunit epsilon; T-complex protein 1, epsilon subunit; epididymis secretory protein Li 69; chaperonin containing TCP1, subunit 5 (epsilon); CCT-epsilon

products gene name :

CCT EPSILON

other gene names :

CCT5; CCT5; CCTE; HEL-S-69; CCT-epsilon; TCP-1-epsilon; CCTE; KIAA0098; TCP-1-epsilon

uniprot entry name :

TCPE_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

PK/29/23/8d

host :

Rat

reactivity :

Bovine, Human, Mouse, Rabbit, Rat

sequence length :

541

specificity :

This item recognises the epsilon polypeptide of the CCT chaperonin molecule complex. The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 1.0 mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Gel Super Shift Assays (GS), Immunoprecipitation (IP), Western Blot (WB)

app notes :

Western Blot: This item detects a band of approximately 60kD in lysates of heat shocked Hela cells. Western Blotting: Minimum Dilution: 1/250; Maximum Dilution: 1/500

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

other info2 :

Buffer Solution: Phosphate buffered saline

products description :

MBS212546 recognises the epsilon polypeptide of the CCT chaperonin molecule complex. The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.

ncbi gi num :

24307939

ncbi acc num :

NP_036205.1

ncbi gb acc num :

NM_012073.3

uniprot acc num :

P48643

ncbi mol weight :

49,526 Da

ncbi pathways :

Assembly Of The Primary Cilium Pathway (1127502); Association Of TriC/CCT With Target Proteins During Biosynthesis Pathway (106247); BBSome-mediated Cargo-targeting To Cilium Pathway (1127506); Cargo Trafficking To The Periciliary Membrane Pathway (1127504); Chaperonin-mediated Protein Folding Pathway (106242); Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway (106243); Folding Of Actin By CCT/TriC Pathway (106246); Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway (106245); Metabolism Of Proteins Pathway (106230); Organelle Biogenesis And Maintenance Pathway (1127494)

ncbi summary :

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. In addition, three pseudogenes of this gene have been identified. [provided by RefSeq, Jun 2010]

uniprot summary :

CCT-epsilon: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Defects in CCT5 are the cause of hereditary sensory neuropathy autosomal recessive with spastic paraplegia (HSNSP). The disease is characterized by spastic paraplegia and progressive distal sensory neuropathy leading to mutilating ulcerations of the upper and lower limbs. Belongs to the TCP-1 chaperonin family. Protein type: Chaperone. Chromosomal Location of Human Ortholog: 5p15.2. Cellular Component: chaperonin-containing T-complex; microtubule; centrosome; cytoplasm; nucleolus; cytosol. Molecular Function: protein binding; unfolded protein binding; beta-tubulin binding; G-protein beta-subunit binding; ATP binding. Biological Process: de novo posttranslational protein folding; cellular protein metabolic process; protein folding; binding of sperm to zona pellucida; response to virus. Disease: Neuropathy, Hereditary Sensory, With Spastic Paraplegia, Autosomal Recessive

size1 :

0.1 mg

price1 :

485 USD

more info or order :

MyBioSource product webpage