antibody

MOUSE ANTI HUMAN DAXX

MBS212521

0.1 mg

300 USD

monoclonal

mouse

nonconjugated

[DAXX-01]

Antibody

MOUSE ANTI HUMAN DAXX

[DAXX]

[death domain-associated protein 6 isoform a; Death domain-associated protein 6; death domain-associated protein 6; CENP-C binding protein; ETS1-associated protein 1; Fas-binding protein; death-associated protein 6; fas death domain-associated protein; death-domain associated protein; Daxx; hDaxx; ETS1-associated protein 1; EAP1; Fas death domain-associated protein]

[DAXX]

[DAXX; DAXX; DAP6; EAP1; BING2; BING2; DAP6; hDaxx; EAP1]

DAXX_HUMAN

Monoclonal

IgG1

[DAXX-01]

Mouse

740

Mouse anti Human DAXX antibody, clone DAXX-01 recognizes human Death domain-associated protein 6, also known as DAXX, ETS1-associated protein 1, EAP1 or Fas death domain-associated protein. DAXX is a 740 amino acid ~81 kDa nucleoplasmic protein. DAXX is a regulator of apoptosis implicated in Fas and TGF beta induced apoptosis. DAXX is predominantly a nuclear protein, where it as a repressor of EtsI and Pax3 transactivation. When in association with ASK1 kinase DAXX translocates from the nucleus to the cytoplasm.

Purified IgG - liquid

IgG concentration 1.0 mg/ml

Store at 4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen, Western Blot (WB)

Where this antibody has been tested for use in a particular technique this does not necessarily exclude it use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appopriate negative/positive controls.

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein A

Immunogen: Recombinant C-terminal DAXX protein (aa 558-740). Buffer Solution: TRIS buffered saline. Target Species: Human

MBS212521 recognises the apoptosis related protein DAXX. DAXX is a regulator of apoptosis implicated in Fas and TGF beta induced apoptosis. DAXX is predominantly a nuclear protein, where it as a repressor of EtsI and Pax3 transactivation. When in association with ASK1 kinase DAXX translocates from the nucleus to the cytoplasm.

215422388

NP_001135441.1

NM_001141969.1

Q9UER7

75,563 Da

Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Androgen Receptor Signaling Pathway (198806); Apoptosis Modulation And Signaling Pathway (198822); FAS Pathway And Stress Induction Of HSP Regulation (198894); HIV-1 Nef: Negative Effector Of Fas And TNF-alpha Pathway (137995); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); IL-6 Signaling Pathway (198864)

This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

DAXX: a transcriptional co-regulatory protein. Proposed to mediate activation of the JNK pathway and apoptosis via ASK1 in response to signaling from FAS and TGF-betaR2. Glucose deprivation activates the ASK1-SEK1-JNK1-HIPK1 pathway, relocalizing Daxx from the nucleus to the cytoplasm, where Daxx binds to ASK1, and subsequently leads to ASK1 oligomerization. Interaction with HSP27 may prevent interaction with TGF-betaR2 and ASK1 and block DAXX-mediated apoptosis. Seems to act as a transcriptional co- repressor and inhibits PAX3 and ETS1 through direct protein- protein interaction. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Two alternatively spliced isoforms have been described. Protein type: Apoptosis; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; Nucleolus. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: heterochromatin; PML body; cytoplasm; nucleolus; cytosol; nucleus; chromosome, pericentric region. Molecular Function: protein homodimerization activity; histone binding; p53 binding; protein N-terminus binding; protein kinase binding; transcription factor binding; protein binding; enzyme binding; androgen receptor binding; ubiquitin protein ligase binding; heat shock protein binding; receptor signaling protein activity; protein kinase activator activity; transcription corepressor activity. Biological Process: chromatin remodeling; nucleosome assembly; transcription, DNA-dependent; cytokinesis after mitosis; regulation of transcription, DNA-dependent; viral reproduction; induction of apoptosis via death domain receptors; regulation of protein ubiquitination; apoptosis; positive regulation of protein kinase activity; androgen receptor signaling pathway; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; activation of JNK activity

0.1 mg

300 USD