MyBioSource

antibody

MOUSE ANTI HUMAN CD25

MBS212517

0.1 mg

230 USD

monoclonal

mouse

human

flow cytometry, FACS

MBS212517

Antibody

MOUSE ANTI HUMAN CD25

CD25

interleukin-2 receptor subunit alpha; Interleukin-2 receptor subunit alpha; interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; TAC antigen; interleukin 2 receptor, alpha; TAC antigen; p55; CD_antigen: CD25

CD25

IL2RA; IL2RA; p55; CD25; IL2R; TCGFR; IDDM10; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA

IL2RA_HUMAN

Monoclonal

IgG1

MEM-181

Mouse

272

Purified. Purified IgG - liquid

IgG concentration 1 mg/ml

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Flow cytometry (FC/FACS)

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution: Phosphate buffered saline. Target Species: Human

Mouse anti Human CD25, clone MEM-181 recognizes the 55kD alpha subunit of the human IL-2 receptor, also known as p55 or TAC antigen, CD25 is a type 1 transmembrane protein with two Sushi domains, also known as short concensus repeats (SCRs) or complement control protein (CCP) modules (Norman et al. 1991) located within its extracellular domain. The IL-2 receptor exists in three forms. A high affinity form consiting of a non-covalently linked heterodimer composed of the alpha subunit (CD25) and the IL-2 receptor beta subunit also known as CD122 or p75, a medium affinity beta subunit (CD122) monomer or a low affinity alpha (CD25) subunit monomer. CD25 is expressed by activated T lymphocytes and activated B lymphocytes responding to antigen or mitogen stimulation. CD25 is also expressed in some thymocytes and oligodendrocytes. In disease, elevated expression of CD25 in noted in a number of chronic inflammatory conditions, tuberculoid leprosy patients demonstrate markedly elevated levels of circulating CD25high FoxP3+ regulatory T cells (T-regs) (Attia et al. 2010). Elevated levels of CD25 antigen expression are often seen in cases of non-Hodgkin 's lymphoma and diffuse large B cell lymphoma (Fujiwara et al.2013).

4557667

NP_000408.1

NM_000417.2

P01589

30,819 Da

Arf6 Trafficking Events Pathway 137954!!Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway 137993!!Calcium Signaling In The CD4+ TCR Pathway 137941!!Cytokine Signaling In Immune System Pathway 366171!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Downstream Signaling In Naive CD8+ T Cells Pathway 138018!!Endocytosis Pathway 102279!!Endocytosis Pathway 102181!!G Beta:gamma Signalling Through PI3Kgamma Pathway 119540

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]

0.1 mg

230 USD