MOUSE ANTI HUMAN PLACENTAL ALKALINE PHOSPHATASE | MyBioSource MBS212484 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN PLACENTAL ALKALINE PHOSPHATASE

catalog :

MBS212484

quantity :

0.2 mg

price :

440 USD

clonality :

monoclonal

host :

mouse

conjugate :

clone name :

H17E2

reactivity :

human

application :

ELISA, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS212484

products type :

Antibody

products full name :

MOUSE ANTI HUMAN PLACENTAL ALKALINE PHOSPHATASE

products short name :

PLACENTAL ALKALINE PHOSPHATASE

other names :

alkaline phosphatase, placental type preproprotein; Alkaline phosphatase, placental type; alkaline phosphatase, placental type; alkaline phosphatase Regan isozyme; alkaline phosphomonoesterase; glycerophosphatase; placental alkaline phosphatase 1; alkaline phosphatase, placental; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1

other gene names :

ALPP; ALPP; ALP; PALP; PLAP; PLAP-1; PLAP; PLAP-1

uniprot entry name :

PPB1_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

H17E2

host :

Mouse

sequence length :

535

specificity :

This item recognises human placental alkaline phosphate, a 67kD isoform expressed in trophoblast cells of normal human placenta, in seminomas and in ovarian carcinoma. The H17E2 antibody has been widely used in immunolocalisation studies of germ cell tumours and may also be used in ELISA based assays for the measurement of soluble PLAP.

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 1.0 mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS)

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections. . Flow Cytometry: Maximum Dilution: 10ug/ml

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

other info2 :

Immunogen: Placental membrane. Histology Positive Control Tissue: Placenta, seminoma. Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Human

products description :

MBS212484 recognises human placental alkaline phosphate, a 67kD isoform expressed in trophoblast cells of normal human placenta, in seminomas and in ovarian carcinoma. The H17E2 antibody has been widely used in immunolocalisation studies of germ cell tumours and may also be used in ELISA based assays for the measurement of soluble PLAP.

ncbi gi num :

94721246

ncbi acc num :

NP_001623.3

ncbi gb acc num :

NM_001632.4

uniprot acc num :

P05187

ncbi mol weight :

57,954 Da

ncbi pathways :

Folate Biosynthesis Pathway (83018); Folate Biosynthesis Pathway (404); Metabolic Pathways (132956); Pyridoxal 5'-phosphate Salvage Pathway (142237)

ncbi summary :

The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015]

uniprot summary :

ALPP: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3 untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]. Protein type: Membrane protein, GPI anchor; EC 3.1.3.1; Cell surface; Membrane protein, integral; Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - folate biosynthesis; Phosphatase (non-protein). Chromosomal Location of Human Ortholog: 2q37. Cellular Component: cell surface; plasma membrane; integral to membrane. Molecular Function: zinc ion binding; alkaline phosphatase activity; magnesium ion binding. Biological Process: dephosphorylation

size1 :

0.2 mg

price1 :

440 USD

more info or order :

MyBioSource product webpage