catalog number :
MBS212475
products full name :
MOUSE ANTI LUCIFERASE
products short name :
LUCIFERASE
other names :
Luciferin 4-monooxygenase; Luciferin 4-monooxygenase; N/A
other gene names :
Luciferase; Luciferase
uniprot entry name :
LUCI_PHOPY
specificity :
Clone LUC-Y detects the expression of the luciferase gene of the North American firefly Photinus pyralis in cells and tissues.
form :
Purified. Purified IgG - liquid
concentration :
IgG concentration 1.0 mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS)*, Western Blot (WB)
app notes :
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Western Blot: This item detects a band of approximately 55 - 60 kDa with a sample of 1ug of purified luciferase from Photinus pyralis on a Western blot. Flow Cytometry: Maximum Dilution: 10ug/ml; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. . Western Blotting: Minimum Dilution: 1/100
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
other info2 :
Immunogen: Recombinant luciferase expressed in E Coli. Fusion Partners: Spleen cells from immunised BALB/c were fused with cells of the SP20 myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Insect
products description :
Clone LUC-Y detects the expression of the luciferase gene of the North American firefly Photinus pyralis in cells and tissues.
ncbi mol weight :
60,745 Da