This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
antibody
product name :
RAT ANTI BrdU
catalog :
MBS212468
quantity :
0.2 mg
price :
475 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
BU1/75 (ICR1)
application :
immunocytochemistry, flow cytometry, FACS
product information
catalog number :
MBS212468
products type :
Antibody
products full name :
RAT ANTI BrdU
products short name :
BrdU
products gene name :
BrdU
clonality :
Monoclonal
isotype :
IgG2a
clone :
BU1/75 (ICR1)
host :
Rat
form :
Purified. Purified IgG - liquid
concentration :
IgG concentration 1.0 mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Immunohistology Paraffin*
app notes :
Flow Cytometry: Use 20ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200; Application Note: See recommended protocol below. . Immunohistology - Paraffin: Minimum Dilution: 1/25; Maximum Dilution: 1/200; Application Note: See recommended protocol below.
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide (NaN3) . Preparation: Purified IgG prepared by affinity chromatography on Protein G
other info2 :
Buffer Solution: Phosphate buffered saline. Target Species: Chemical
products description :
Rat anti BrdU antibody clone BU1/75 (ICR1), recognizes bromodeoxyuridine (known as BrdU or BrdUrd). Rat anti BrdU antibody clone BU1/75 (ICR1) reacts with BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. Rat anti BrdU antibody, clone BU1/75 (ICR1) cross reacts with chlorodeoxyuridine (CldU) but does not cross react with thymidine or iododeoxyuridine (Aten et al. 1992). BrdU, IdU and CldU are analogs of thymidine, they can incorporate into DNA during DNA synthesis replacing thymidine.Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004) Clone BU1/75 (ICR1) has been used to detect CldU to study the speed of DNA replication fork (Bugler et al. 2010), in the detection of CldU label retaining stem cells (Kimoto et al. 2008) and label retaining neurons (Murata et al. 2011).
size1 :
0.2 mg
price1 :
475 USD
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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