antibody

MOUSE ANTI HUNTINGTIN

MBS212463

0.1 mg

445 USD

monoclonal

mouse

nonconjugated

HDA3E10

human, mouse

western blot, immunoprecipitation

Antibody

MOUSE ANTI HUNTINGTIN

HUNTINGTIN

huntingtin; Huntingtin; huntingtin; huntington disease protein; huntingtin; Huntington disease protein; HD protein

HTT; HTT; HD; IT15; HD; IT15; HD protein

HD_HUMAN

Monoclonal

IgG2a

HDA3E10

Mouse

Human, Mouse, Rabbit

3144

Purified. Purified IgG - liquid

IgG concentration 1.0mg/ml

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen*, Immunoprecipitation (IP), Western Blot (WB)

Immunohistology - Frozen: Application Note: Increased cytoplasmic staining, relative to nuclear, has been reported using formaldehyde as a fixative compared with acetone/methanol, see Wilkinson et al.

Perservative Stabilisers: 0.09% Sodium Azide

Histology Positive Control Tissue: Brain. Buffer Solution: Phosphate buffered saline

Mouse anti Huntingtin antibody, clone HDA3E10 reacts with an epitope corresponding to the HDA region (amino acids 1171 - 1177) of the huntingtin protein. Mouse anti Huntingtin antibody, clone HDA3E10 detects a 350KD band on western blots but also detects smaller degradation products of huntingtin. The combined use of clone HDA3E10 (MBS212463) HDC8A4 (MBS212465) and HDB4E10 (MBS212464) demonstrate that huntingtin is enriched in neuronal cells in the brain.

90903231

NP_002102.4

NM_002111.7

P42858

347,603 Da

Direct P53 Effectors Pathway 137939!!EGFR1 Signaling Pathway 198782!!Huntington's Disease Pathway 83100!!Huntington's Disease Pathway 512

Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression. [provided by RefSeq, Jul 2008]

0.1 mg

445 USD