MyBioSource

antibody

MOUSE ANTI HUMAN HLA G

MBS212456

0.2 mg

530 USD

monoclonal

mouse

human

immunocytochemistry, ELISA, FACS, flow cytometry, enzyme immunoassay

MBS212456

Antibody

MOUSE ANTI HUMAN HLA G

HLA G

HLA class I histocompatibility antigen, alpha chain G; HLA class I histocompatibility antigen, alpha chain G; HLA class I histocompatibility antigen, alpha chain G; HLA G antigen; HLA class I molecule; HLA-G histocompatibility antigen, class I, G; MHC class I antigen G; b2 microglobulin; major histocompatibility complex, class I, G; HLA G antigen; MHC class I antigen G

HLA G

HLA-G; HLA-G; MHC-G; HLA-6.0; HLAG

HLAG_HUMAN

Monoclonal

IgG1

MEM-G/9

Mouse

338

Purified. Purified IgG - liquid

IgG concentration 1.0mg/ml

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS), Immunofluorescence (IF)

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein A

Immunogen: Recombinant human HLA-G refolded with beta 2 microglobulin. Fusion Partners: Spleen cells from immunised Balb/c mice were fused with myeloma cells. Buffer Solution: Phosphate buffered saline. Target Species: Human

Mouse anti Human HLA G antibody, clone MEM-G/9 recognizes human HLA-G, a non-classical major histocompatibility complex (MHC) molecule. HLA-G expression is restricted to trophoblast cells and some medullary thymic epithelial cells. Several isoforms of the HLA-G molecule exist, which include the membrane bound isoforms HLA-G1 - G4 and soluble isoforms HLA-G5 - G7. Clone MEM-G/9 specifically recognizes surface expressed native HLA-G1, when associated with beta 2 microglobulin, but not does recognize the isoforms HLA-G2, G3 and G4. CMouse anti Human HLA G antibody, clone MEM-G/9 has also been reported to recognize the soluble isoform HLA-G5.

4504415

NP_002118.1

NM_002127.5

P17693

38,224 Da

Adaptive Immune System Pathway 366160!!Allograft Rejection Pathway 920963!!Allograft Rejection Pathway 83123!!Allograft Rejection Pathway 535!!Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway 366163!!Antigen Processing And Presentation Pathway 83074!!Antigen Processing And Presentation Pathway 485!!Antigen Processing-Cross Presentation Pathway 477122!!Autoimmune Thyroid Disease Pathway 83121!!Autoimmune Thyroid Disease Pathway 533

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

0.2 mg

530 USD