MOUSE ANTI HUMAN CD151 | MyBioSource MBS212354 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN CD151

catalog :

MBS212354

quantity :

0.2 mg

price :

415 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

11G5a

reactivity :

human

application :

western blot, ELISA, immunoprecipitation, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS212354

products type :

Antibody

products full name :

MOUSE ANTI HUMAN CD151

products short name :

CD151

other names :

CD151 antigen; CD151 antigen; CD151 antigen; CD151 antigen (Raph blood group); hemidesmosomal tetraspanin CD151; membrane glycoprotein SFA-1; platelet surface glycoprotein gp27; platelet-endothelial cell tetraspan antigen 3; platelet-endothelial tetraspan antigen 3; tetraspanin-24; tspan-24; CD151 molecule (Raph blood group); GP27; Membrane glycoprotein SFA-1; Platelet-endothelial tetraspan antigen 3; PETA-3; Tetraspanin-24; Tspan-24; CD_antigen: CD151

products gene name :

CD151

other gene names :

CD151; CD151; GP27; MER2; RAPH; SFA1; PETA-3; TSPAN24; TSPAN24; PETA-3; Tspan-24

uniprot entry name :

CD151_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

11G5a

host :

Mouse

sequence length :

253

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 1.0 mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood . Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100. Immunohistology - Paraffin: Maximum Dilution: 1/100; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

other info2 :

Histology Positive Control Tissue: Tonsil/spleen. Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Human

products description :

Mouse anti Human CD151 antibody, clone 11G5a recognizes the human CD151 cell surface antigen, also known as PETA-3. CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen (Crew et al. 2004). It should be noted that CD151 is very closely associated with the alpha3 beta1 integrin in cells and co-immunoprecipitation may occur even in quite stringent conditions (Yauch. et al. 1998).

ncbi gi num :

87159824

ncbi acc num :

NP_001034579.1

ncbi gb acc num :

NM_001039490.1

uniprot acc num :

P48509

ncbi mol weight :

28,295 Da

ncbi pathways :

Alpha6-Beta4 Integrin Signaling Pathway (198807); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Collagen Formation Pathway (645288); Extracellular Matrix Organization Pathway (576262); Type I Hemidesmosome Assembly Pathway (160970)

ncbi summary :

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells. Multiple alternatively spliced transcript variants that encode the same protein have been described for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

CD151: Essential for the proper assembly of the glomerular and tubular basement membranes in kidney. Defects in CD151 are the cause of nephropathy with pretibial epidermolysis bullosa and deafness (NPEBD). NPEBD is characterized by the association of hereditary nephritis, epidermolysis bullosa, deafness, and beta-thalassemia minor. Belongs to the tetraspanin (TM4SF) family. Protein type: Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; cytosol. Molecular Function: integrin binding; protein binding. Biological Process: T cell proliferation; cell migration; hemidesmosome assembly; extracellular matrix organization and biogenesis; cell adhesion. Disease: Raph Blood Group System; Nephropathy With Pretibial Epidermolysis Bullosa And Deafness

size1 :

0.2 mg

price1 :

415 USD

more info or order :

MyBioSource product webpage