catalog number :
MBS212300
products full name :
MOUSE ANTI HUMAN MYELOPEROXIDASE
products short name :
MYELOPEROXIDASE
other names :
myeloperoxidase; Myeloperoxidase; myeloperoxidase; myeloperoxidase
other gene names :
MPO; MPO; MPO
uniprot entry name :
PERM_HUMAN
form :
Purified. Purified IgG - liquid
concentration :
IgG concentration 1.0 mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS)*, Immunohistology Paraffin, Western Blot (WB)*
app notes :
Immunohistology - Frozen: Minimum Dilution: 1/1000; Maximum Dilution: 1/5000;. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. . Immunohistology - Paraffin: Minimum Dilution: 1/500; Maximum Dilution: 1/1000;. Western Blotting: Application Note: 2C7 recognises myeloperoxidase under non-reducing conditions, see Audrian et al. for details
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein G
other info2 :
Histology Positive Control Tissue: Bone marrow. Buffer Solution: Phosphate buffered saline. Target Species: Human
products description :
Mouse anti Human Myeloperoxidase antibody, clone 2C7 recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulphide bond. Mouse anti Human Myeloperoxidase antibody, clone 2C7 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. Mouse anti Human Myeloperoxidase antibody, clone 2C7 also recognizes recombinant MPO in Western blots and weakly in ELISA. Mouse anti Human Myeloperoxidase antibody, clone 2C7 may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation.
ncbi acc num :
NP_000241.1
ncbi gb acc num :
NM_000250.1
ncbi mol weight :
87,248 Da
ncbi pathways :
C-MYB Transcription Factor Network Pathway 138073!!Folate Metabolism Pathway 198833!!IL23-mediated Signaling Events Pathway 138000!!Phagosome Pathway 153910!!Phagosome Pathway 153859!!Selenium Pathway 198825!!Transcriptional Misregulation In Cancer Pathway 523016!!Transcriptional Misregulation In Cancer Pathway 522987!!Vitamin B12 Metabolism Pathway 920974!!Amb2 Integrin Signaling Pathway 137945
ncbi summary :
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]