catalog number : 
MBS212298
products full name : 
MOUSE ANTI CYCLIN D1
products short name : 
CYCLIN D1
other names : 
G1/S-specific cyclin-D1; G1/S-specific cyclin-D1; G1/S-specific cyclin-D1; B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL-1 oncogene; PRAD1 oncogene; cyclin D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene
products gene name : 
CYCLIN D1
other gene names : 
CCND1; CCND1; BCL1; PRAD1; U21B31; D11S287E; BCL1; PRAD1; BCL-1
uniprot entry name : 
CCND1_HUMAN
form : 
Purified. Purified IgG - liquid
concentration : 
IgG concentration 1.0 mg/ml
storage stability : 
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.  Shelf Life: 18 months from date of despatch.
tested application : 
Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS)*, Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)
app notes : 
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.   Flow Cytometry:  Maximum Dilution: Neat; Application Note:  Membrane permeabilization is required for this application. MyBioSource recommend the use of Leucoperm  .  Immunohistology - Paraffin:  Minimum Dilution: 1/1000; Maximum Dilution: 1/10000; Application Note:  This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. .  Western Blotting:  Maximum Dilution: 1/500
other info1 : 
Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
other info2 : 
Histology Positive Control Tissue: Small intestine, colon. Buffer Solution: Phosphate buffered saline.  Target Species: Human
products description : 
Mouse anti Cyclin D1 antibody, clone CD1.1 recognizes cyclin D1, a 295 amino acid, 36kDa nuclear protein which belongs to the highly conserved cyclin family. D type cyclins are predominantly expressed in the G1 phase of the cell cycle. Cyclin D1 study is of interest in various forms of malignancy and over expression has been linked to poor prognosis (Bartkova et al. 1995).
ncbi acc num : 
NP_444284.1
ncbi gb acc num : 
NM_053056.2
ncbi mol weight : 
33,729 Da
ncbi pathways : 
AMPK Signaling Pathway  989139!!AMPK Signaling Pathway  992181!!ATF-2 Transcription Factor Network Pathway  138006!!Acute Myeloid Leukemia Pathway  83117!!Acute Myeloid Leukemia Pathway  529!!Androgen Receptor Signaling Pathway  198806!!Bladder Cancer Pathway  83115!!Bladder Cancer Pathway  527!!C-MYB Transcription Factor Network Pathway  138073!!Cell Cycle Pathway  530733
ncbi summary : 
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]