catalog number :
MBS212272
products full name :
MOUSE ANTI MDM2
products short name :
MDM2
other names :
E3 ubiquitin-protein ligase Mdm2 isoform h; E3 ubiquitin-protein ligase Mdm2; E3 ubiquitin-protein ligase Mdm2; MDM2 oncogene, E3 ubiquitin protein ligase; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; double minute 2, human homolog of; p53-binding protein; oncoprotein Mdm2; MDM2 proto-oncogene, E3 ubiquitin protein ligase; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2
products gene name :
MDM2
other gene names :
MDM2; MDM2; HDMX; hdm2; ACTFS; Hdm2
uniprot entry name :
MDM2_HUMAN
form :
Purified. Purified IgG - liquid
concentration :
IgG concentration 1.0 mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Immunohistology Frozen, Immunoprecipitation (IP), Immunohistology Paraffin*, Immunohistology Resin (RE)
app notes :
Immunohistology - Paraffin: Minimum Dilution: 1/200; Maximum Dilution: 1/500; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. EDTA pH8.0 is recommended for this purpose.
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein G
other info2 :
Immunogen: Synthetic peptide, CSRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2. Histology Positive Control Tissue: Breast Carcinoma. Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Human
products description :
Mouse anti MDM2 antibody, clone SMP14 recognizes the 90kDa MDM2 protein, which forms a complex with p53 leading to p53 inhibition. MDM2 also acts to stimulate cell proliferation via its induction of transcription factors such as E2F1 and DPI. MDM2 is over expressed in a range of human malignancies, including soft tissue sarcomas, breast cancer and bladder cancer.
ncbi acc num :
NP_001138811.1
ncbi gb acc num :
NM_001145339.2
ncbi mol weight :
55,991 Da
ncbi pathways :
AKT Phosphorylates Targets In The Cytosol Pathway 106475!!Adaptive Immune System Pathway 366160!!Androgen Receptor Signaling Pathway 198806!!Apoptosis Pathway 198797!!Aurora A Signaling Pathway 137925!!Bladder Cancer Pathway 83115!!Bladder Cancer Pathway 527!!Cell Cycle Pathway 530733!!Cell Cycle Checkpoints Pathway 105739!!Cell Cycle Pathway 198811
ncbi summary :
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]