catalog number :
MBS212215
products full name :
MOUSE ANTI HUMAN CD154 (CD40L)
products short name :
CD154
other names :
CD40 ligand; CD40 ligand; CD40 ligand; CD40 antigen ligand; CD40-L; T-B cell-activating molecule; T-cell antigen Gp39; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5; CD40 ligand; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD_antigen: CD154Cleaved into the following 2 chains:CD40 ligand, membrane form; CD40 ligand, soluble form
products gene name :
CD154
other gene names :
CD40LG; CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L; CD40L; TNFSF5; TRAP; CD40-L; TRAP
uniprot entry name :
CD40L_HUMAN
form :
Purified. Purified IgG - liquid
concentration :
IgG concentration 1.0 mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Flow cytometry (FC/FACS)
app notes :
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/50
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide (NaN3). 0.1% Bovine Serum Albumin. Preparation: Purified IgG prepared by affinity chromatography on Protein A from ascites
other info2 :
Buffer Solution: Phosphate buffered saline. Target Species: Human
products description :
Mouse anti Human CD154 antibody, clone TRAP-1 recognises the human CD40 ligand, also known as CD154, TNF-related activation protein (TRAP) or T-cell antigen Gp39. CD154 is a 261 amino acid ~32kDa single pass, type-1 transmembrane glycoprotein (UniProt: P29965). CD154 is expressed on activated T lymphocytes, predominantly CD4 +ve and also on some basophils and mast cells. Mouse anti Human CD154 antibody, clone TRAP-1 binds to CD154 at an epitope distinct from the CD40 binding site (Kroczek et al. 1994).
ncbi acc num :
NP_000065.1
ncbi gb acc num :
NM_000074.2
ncbi mol weight :
29,274 Da
ncbi pathways :
Adaptive Immune System Pathway 366160!!Allograft Rejection Pathway 920963!!Allograft Rejection Pathway 83123!!Allograft Rejection Pathway 535!!Asthma Pathway 83120!!Asthma Pathway 532!!Autoimmune Thyroid Disease Pathway 83121!!Autoimmune Thyroid Disease Pathway 533!!CD40/CD40L Signaling Pathway 138061!!Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway 137993
ncbi summary :
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]