catalog number :
MBS212125
products full name :
MOUSE ANTI HEPATITIS B X ANTIGEN
products short name :
HEPATITIS B X ANTIGEN
other names :
Protein X; Protein X; HBx; Peptide X; pX
uniprot entry name :
X_HBVD3
form :
Purified. Purified IgG - liquid
concentration :
IgG concentration 1.0 mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Immunohistology Frozen, ELISA (EIA), Immunohistology Paraffin, Western Blot (WB)
app notes :
Immunohistology - Frozen: Maximum Dilution: 1/100;. Immunohistology - Paraffin: Maximum Dilution: 1/100;. Western Blotting: Minimum Dilution: 1/100; Maximum Dilution: 1/500
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide. 0.1% Bovine Serum Albumin. Preparation: Purified IgG prepared by affinity chromatography on Protein G.
other info2 :
Histology Positive Control Tissue: Liver Carcinoma/Hepatitis B infected liver. Target Species: Viral
products description :
Mouse anti Hepatitis B-X antibody, clone 3F6-G10 recognizes the HB-X antigen of hepatitis virus. The Hepatitis B X antigen is a 154 amino acid ~17 kDa multifunctional protein involved in the development of liver chirrosis and hepatocellular carcinoma (UniProt: P03165). Clone 3F6-G10 was produced by immunization of mice with a "HB-X- Protein A" fusion construct and subsequent screening of hybridoma products against a "HB-X-GST" fusion construct (Marczinovits et al. 1997). Mouse anti Hepatitis B-X antibody, clone 3F6-G10 has been used succesfully for the detection of the hepatitis B X antigen by immunohistochemistry in formalin fixed, paraffin embedded material, also by western blotting against the immunizing and screening fusion proteins (P l et al. 2001). Subsequently clone 3F6-G10 has been used as a capture reagent in a sensitive sandwich ELISA and bead based flow assay for the quantitative assessment of HbX antigen in human sera (P l et al. 2005). Fine epitope mapping by phage library screening indicates that the epitope recognized by Mouse anti Hepatitis B-X antibody, clone 3F6-G10 lies between amino acids 88 and 93 of the X antigen, a result subsequently confirmed by peptide ELISA (P l et al. 2003).
ncbi mol weight :
16,618 Da