MOUSE ANTI HUMAN SNAP-25 | MyBioSource MBS212090 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN SNAP-25

catalog :

MBS212090

quantity :

0.2 mg

price :

405 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SP12

reactivity :

human, rat

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS212090

products type :

Antibody

products full name :

MOUSE ANTI HUMAN SNAP-25

products short name :

SNAP-25

other names :

synaptosomal-associated protein 25 isoform SNAP25A; Synaptosomal-associated protein 25; synaptosomal-associated protein 25; SUP; resistance to inhibitors of cholinesterase 4 homolog; super protein; synaptosomal-associated protein, 25kD; synaptosomal-associated protein, 25kDa; Super protein; SUP; Synaptosomal-associated 25 kDa protein

products gene name :

SNAP-25

other gene names :

SNAP25; SNAP25; RIC4; SEC9; SNAP; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2; SNAP; SNAP-25; SUP

uniprot entry name :

SNP25_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

SP12

host :

Mouse

reactivity :

Gerbil, Hamster, Pig, Rat

sequence length :

206

form :

Purified. Purified IgG - liquid

concentration :

IgG concentration 1.0 mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

ELISA (EIA), Immunohistology Paraffin, Western Blot (WB)

app notes :

Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Immunohistology - Paraffin: Minimum Dilution: 1/2000; Maximum Dilution: 1/5000. Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/5000

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

other info2 :

Histology Positive Control Tissue: Brain. Buffer Solution: Phosphate buffered saline. Target Species: Human

products description :

Mouse anti Human SNAP-25 antibody, clone SP12 recognizes the human pre-synaptic protein, SNAP-25, also known as Synaptosomal-associated protein 25, Super protein (SUP) or Synaptosomal-associated 25 kDa protein. SNAP-25 is a 206 amino acid presynaptic protein of ~25kDa containing two t-SNARE coiled-coil homology domains. Mouse anti human SNAP-25 antibody, clone SP12 will recognize SNAP-25 fusion protein from COS cells, but not the fusion protein from bacterial systems. Mouse anti Human SNAP-25 antibody, clone SP12 has been used to study the distribution of synaptic changes in the hippocampus of patients with medically refractory temporal lobe epilepsy. (Honer et al. 1994).

ncbi gi num :

18765733

ncbi acc num :

NP_003072.2

ncbi gb acc num :

NM_003081.3

uniprot acc num :

P60880

ncbi mol weight :

23,336 Da

ncbi pathways :

Acetylcholine Neurotransmitter Release Cycle Pathway (106525); Disease Pathway (530764); Dopamine Neurotransmitter Release Cycle Pathway (106523); Effects Of Botulinum Toxin Pathway (138028); GABA Synthesis, Release, Reuptake And Degradation Pathway (187174); Glutamate Neurotransmitter Release Cycle Pathway (106522); Insulin Signaling Pathway (198845); Insulin Secretion Pathway (777534); Integration Of Energy Metabolism Pathway (106096); Metabolism Pathway (477135)

ncbi summary :

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

SNAP-25: a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Part of the SNARE core complex containing SNAP25, VAMP2 and syntaxin 1A. Associates with proteins involved in vesicle docking and membrane fusion. Two alternatively spliced isoforms have been described. Protein type: Vesicle. Chromosomal Location of Human Ortholog: 20p12-p11.2. Cellular Component: presynaptic membrane; SNARE complex; neuron projection; growth cone; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; trans-Golgi network; cell junction; cytosol; vesicle. Molecular Function: SNAP receptor activity; protein binding; syntaxin-1 binding; calcium-dependent protein binding. Biological Process: synaptic vesicle fusion to presynaptic membrane; synaptic transmission; synaptic vesicle exocytosis; glutamate secretion; neurotransmitter secretion; energy reserve metabolic process; neurotransmitter uptake; pathogenesis; regulation of insulin secretion; synaptic vesicle priming; synaptic vesicle docking during exocytosis. Disease: Myasthenic Syndrome, Congenital, 18

size1 :

0.2 mg

price1 :

405 USD

more info or order :

MyBioSource product webpage