antibody

MOUSE ANTI HUMAN THYROID STIMULATING HORMONE RECEPTOR

MBS212076

0.2 mg

610 USD

monoclonal

mouse

nonconjugated

2C11

western blot, ELISA, immunoprecipitation, flow cytometry, FACS, enzyme immunoassay

Antibody

MOUSE ANTI HUMAN THYROID STIMULATING HORMONE RECEPTOR

THYROID STIMULATING HORMONE RECEPTOR

thyrotropin receptor isoform 1; Thyrotropin receptor; thyrotropin receptor; seven transmembrane helix receptor; thyroid stimulating hormone receptor, isoform 2; thyrotropin receptor-I, hTSHR-I; thyroid stimulating hormone receptor; Thyroid-stimulating hormone receptor; TSH-R

TSHR; TSHR; LGR3; CHNG1; hTSHR-I; LGR3; TSH-R

TSHR_HUMAN

Monoclonal

IgG1

2C11

Mouse

764

Purified. Purified IgG - liquid

IgG concentration 1.0 mg/ml

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

ELISA (EIA), Flow cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistology Paraffin, Western Blot (WB)

Immunoprecipitation: Minimum Dilution: 5ug/ml; Maximum Dilution: 10ug/ml;. Western Blotting: Minimum Dilution: 5ug/ml; Maximum Dilution: 10ug/ml

Perservative Stabilisers: 0.09% Sodium Azide. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution: Phosphate buffered saline. Target Species: Human

Mouse anti Human thyroid stimulating hormone receptor antibody, clone 2C11 recognizes the human thyroid stimulating hormone receptor (TSHR) otherwise known as the thyrotropin receptor. TSHR is an important molecule in controlling the growth and function of the normal thyroid. Mouse anti Human thyroid stimulating receptor antibody recognises both native and denatured TSH receptor (binding to an epitope at the carboxy terminus between amino acids 354 and 359). It does inhibit binding of TSH. No cross reactivity has been observed with related LH and FSH receptors. Clone 2C11 recognises the mutant TSH receptor known as I167N as well as the wild type molecule (Costagliola et al. 1998)

64085121

NP_000360.2

NM_000369.2

P16473

30,800 Da

Arf6 Signaling Events Pathway (138034); Arf6 Trafficking Events Pathway (137954); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020)

The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]

TSHR: Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5). Defects in TSHR are found in patients affected by hyperthyroidism with different etiologies. Somatic, constitutively activating TSHR mutations and/or constitutively activating G(s)alpha mutations have been identified in toxic thyroid nodules (TTNs) that are the predominant cause of hyperthyroidism in iodine deficient areas. These mutations lead to TSH independent activation of the cAMP cascade resulting in thyroid growth and hormone production. TSHR mutations are found in autonomously functioning thyroid nodules (AFTN), toxic multinodular goiter (TMNG) and hyperfunctioning thyroid adenomas (HTA). TMNG encompasses a spectrum of different clinical entities, ranging from a single hyperfunctioning nodule within an enlarged thyroid, to multiple hyperfunctioning areas scattered throughout the gland. HTA are discrete encapsulated neoplasms characterized by TSH- independent autonomous growth, hypersecretion of thyroid hormones, and TSH suppression. Defects in TSHR are also a cause of thyroid neoplasms (papillary and follicular cancers). Autoantibodies against TSHR are directly responsible for the pathogenesis and hyperthyroidism of Graves disease. Antibody interaction with TSHR results in an uncontrolled receptor stimulation. Defects in TSHR are the cause of congenital hypothyroidism non-goitrous type 1 (CHNG1); also known as congenital hypothyroidism due to TSH resistance. CHNG1 is a non-autoimmune condition characterized by resistance to thyroid- stimulating hormone (TSH) leading to increased levels of plasma TSH and low levels of thyroid hormone. CHNG1 presents variable severity depending on the completeness of the defect. Most patients are euthyroid and asymptomatic, with a normal sized thyroid gland. Only a subset of patients develop hypothyroidism and present a hypoplastic thyroid gland. Defects in TSHR are the cause of familial gestational hyperthyroidism (HTFG). HTFG is a condition characterized by abnormally high levels of serum thyroid hormones occurring during early pregnancy. Defects in TSHR are the cause of hyperthyroidism non- autoimmune (HTNA). It is a condition characterized by abnormally high levels of serum thyroid hormones, thyroid hyperplasia, goiter and lack of anti-thyroid antibodies. Typical features of Graves disease such as exophthalmia, myxedema, antibodies anti-TSH receptor and lymphocytic infiltration of the thyroid gland are absent. Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR. Chromosomal Location of Human Ortholog: 14q31. Cellular Component: integral to plasma membrane; plasma membrane; receptor complex. Molecular Function: protein binding; thyroid-stimulating hormone receptor activity. Biological Process: G-protein signaling, coupled to cyclic nucleotide second messenger; G-protein coupled receptor protein signaling pathway; cell-cell signaling; positive regulation of cell proliferation. Disease: Hyperthyroidism, Nonautoimmune; Hypothyroidism, Congenital, Nongoitrous, 1; Hyperthyroidism, Familial Gestational

0.2 mg

610 USD