MOUSE ANTI HUMAN ALPHA FETOPROTEIN | MyBioSource MBS211649 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN ALPHA FETOPROTEIN

catalog :

MBS211649

quantity :

1 mg

price :

675 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[2I9/2]

reactivity :

human

application :

ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein as a detection reagent with human alpha fetoprotein as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are both displayed at 1:2 dilution.

image 2 :

Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein (MBS211649) as a detection reagent with human alpha fetoprotein as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are both displayed at 1:2 dilution.

image 3 :

Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein (MBS211395) as a detection reagent with human alpha fetoprotein as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are both displayed at 1:2 dilution.

product information

catalog number :

MBS211649

products type :

Antibody

products full name :

MOUSE ANTI HUMAN ALPHA FETOPROTEIN

products short name :

[ALPHA FETOPROTEIN]

other names :

[alpha-fetoprotein; Alpha-fetoprotein; alpha-fetoprotein; alpha-1-fetoprotein; alpha-fetoglobulin; alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin]

other gene names :

[AFP; AFP; AFPD; FETA; HPAFP; HPAFP]

uniprot entry name :

FETA_HUMAN

clonality :

Monoclonal

isotype :

IgG

clone :

[2I9/2]

host :

Mouse

sequence length :

609

specificity :

This item recognises alpha fetoprotein (AFP), a member of the albuminoid superfamily. It is a 68kD plasma protein found in fetuses over four weeks old, produced by the yolk sac and the fetal liver, with the highest concentration reached during the 12th to 16th week of gestation. It binds and transports various ligands such as bilirubin, fatty acids, steroids, heavy metals and various drugs. After birth, plasma levels drop to trace amounts in healthy adults. Elevated AFP levels are indicative of certain cancers, particularly hepatocellular carcinoma, cancer of the testes or ovaries or a malignant teratoma. During pregnancy increased levels of AFP may indicate neural tube defects such as spina bifida, while decreased levels could indicate Down's syndrome. This item does not cross-react with human albumin and other human serum proteins.

form :

Purified. Ig fraction - liquid

concentration :

IgG concentration 1.0 mg/ml

storage stability :

Store at 4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

ELISA (EIA)

image1 heading :

Testing Data

image2 heading :

Testing Data

image3 heading :

Testing Data

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide (NaN3). Preparation: Ig fraction prepared by chromatography

other info2 :

Immunogen: Human fetal cord serum. Buffer Solution: Phosphate buffered saline. Target Species: Human

products description :

MBS211649 recognises alpha fetoprotein (AFP), a member of the albuminoid superfamily. It is a 68kD plasma protein found in fetuses over four weeks old, produced by the yolk sac and the fetal liver, with the highest concentration reached during the 12th to 16th week of gestation. It binds and transports various ligands such as bilirubin, fatty acids, steroids, heavy metals and various drugs. After birth, plasma levels drop to trace amounts in healthy adults. Elevated AFP levels are indicative of certain cancers, particularly hepatocellular carcinoma, cancer of the testes or ovaries or a malignant teratoma. During pregnancy increased levels of AFP may indicate neural tube defects such as spina bifida, while decreased levels could indicate Down's syndrome. MBS211649 does not cross-react with human albumin and other human serum proteins.

ncbi gi num :

4501989

ncbi acc num :

NP_001125.1

ncbi gb acc num :

NM_001134.2

uniprot acc num :

P02771

ncbi pathways :

Direct P53 Effectors Pathway (137939); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Glucocorticoid Receptor Regulatory Network Pathway (138014); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388)

ncbi summary :

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

uniprot summary :

AFP: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Dimeric and trimeric forms have been found in addition to the monomeric form. Plasma. Synthesized by the fetal liver and yolk sac. Belongs to the ALB/AFP/VDB family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 4q13.3. Cellular Component: extracellular space; cytoplasm. Molecular Function: metal ion binding. Biological Process: transport; progesterone metabolic process; ovulation from ovarian follicle. Disease: Alpha-fetoprotein Deficiency; Alpha-fetoprotein, Hereditary Persistence Of

size1 :

1 mg

price1 :

675 USD

more info or order :

MyBioSource product webpage