antibody

MOUSE ANTI HUMAN ANKYRIN G

MBS211304

0.1 mg

1690 USD

monoclonal

mouse

nonconjugated

463

human, mouse, rat, dog

western blot, immunocytochemistry

Antibody

MOUSE ANTI HUMAN ANKYRIN G

ANKYRIN G

ankyrin-3 isoform 2; Ankyrin-3; ankyrin-3; ankyrin G119; ankyrin 3, node of Ranvier (ankyrin G); Ankyrin-G

ANK3; ANK3; MRT37; ANKYRIN-G; ANK-3

ANK3_HUMAN

Monoclonal

IgG1

463

Mouse

Dog, Mouse, Rat

1001

This item is specific for ankyrin G and reacts with a region within the spectrin binding domain. In Western blotting it detects the 190 kDa isoform. Ankyrins are a family of spectrin-binding protein that couple the spectrin/actin cytoskeletal network to the cytoplasmic domains of integral membrane proteins which include channels such as the anion exchanger, voltage-dependent sodium channel and the Na/K ATPase. 3 forms of ankyrin G exists, 190 kDa, 270 kDa and 480 kDa, that are localized in the axonal intial segments and the nodes of Ranvier of myelinated axons where fluxes of action potential occur and ion channels are enriched.

Purified. Purified IgG - liquid

IgG concentration 0.1 mg/ml

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Immunohistology Frozen, Immunofluorescence (IF), Western Blot (WB)

Immunohistology - Frozen: Maximum Dilution: 1-2 ug/ml;. Immunofluorescence: Maximum Dilution: 1-2 ug/ml;. Western Blotting: Maximum Dilution: 2-5 ug/ml

Perservative Stabilisers: 0.09% Sodium Azide (NaN3). 0.2% Gelatin

Immunogen: A synthetic peptide corresponding to amino acids from the spectrin-binding domain of human ankyrin G. Histology Positive Control Tissue: Mouse or rat brain. Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the P3X63/Ag 8.653 mouse myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Human

MBS211304 is specific for ankyrin G and reacts with a region within the spectrin binding domain. In Western blotting it detects the 190 kDa isoform.Ankyrins are a family of spectrin-binding protein that couple the spectrin/actin cytoskeletal network to the cytoplasmic domains of integral membrane proteins which include channels such as the anion exchanger, voltage-dependent sodium channel and the Na/K ATPase. 3 forms of ankyrin G exists, 190 kDa, 270 kDa and 480 kDa, that are localized in the axonal intial segments and the nodes of Ranvier of myelinated axons where fluxes of action potential occur and ion channels are enriched.

32967599

NP_001140.2

NM_001149.3

Q12955

160,947 Da

Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); Interaction Between L1 And Ankyrins Pathway (161005); L1CAM Interactions Pathway (161003); Proteoglycans In Cancer Pathway (782000); Proteoglycans In Cancer Pathway (782054)

Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact, and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 3 is an immunologically distinct gene product from ankyrins 1 and 2, and was originally found at the axonal initial segment and nodes of Ranvier of neurons in the central and peripheral nervous systems. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

ANK3: In skeletal muscle, required for costamere localization of DMD and betaDAG1. Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. Genetic variations in ANK3 are associated with autism spectrum disorders susceptibility. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 10q21. Cellular Component: Golgi apparatus; tight junction; costamere; cell surface; sarcoplasmic reticulum; endoplasmic reticulum; basolateral plasma membrane; lysosome; paranode region of axon; T-tubule; dendrite; Z disc; postsynaptic membrane; plasma membrane; basal plasma membrane; neuromuscular junction; sarcolemma; lateral plasma membrane. Molecular Function: protein binding, bridging; protein binding; spectrin binding; structural constituent of cytoskeleton; cadherin binding; cytoskeletal protein binding. Biological Process: axon guidance; regulation of potassium ion transport; cytoskeletal anchoring; generation of action potential; signal transduction; positive regulation of homotypic cell-cell adhesion; establishment of protein localization; cytokinesis after mitosis; plasma membrane organization and biogenesis; axonogenesis; Golgi to plasma membrane protein transport; positive regulation of membrane potential; neuromuscular junction development. Disease: Mental Retardation, Autosomal Recessive 37

0.1 mg

1690 USD