antibody

MOUSE ANTI HUMAN CD4:Low Endotoxin

MBS211194

0.5 mg

550 USD

monoclonal

mouse

nonconjugated

RPA-T4

flow cytometry, FACS

Antibody

MOUSE ANTI HUMAN CD4:Low Endotoxin

CD4

T-cell surface glycoprotein CD4 isoform 1; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4; CD4 antigen (p55); CD4 receptor; T-cell surface antigen T4/Leu-3; CD4 molecule; T-cell surface antigen T4/Leu-3; CD_antigen: CD4

CD4

CD4; CD4; CD4mut

CD4_HUMAN

Monoclonal

IgG1

RPA-T4

Mouse

458

Mouse anti human CD4 antibody, clone RPA-T4 recognizes human CD4, a 55kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages. Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognize an epitope within domain 1, of the extracellular region, of the CD4 molecule. Mouse anti human CD4 antibody, clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation, see Piatier-Tonneau et al , 1997 for details. MBS recommend the use of Mouse anti Human CD4:Low Endotoxin for this purpose.

Low Endotoxin. Purified IgG - liquid

IgG concentration 1.0 mg/ml

Store at -20 degree C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch. Shipping: Ships at 4 degree C.

Immunohistology Frozen*, Flow cytometry (FC/FACS), Functional Assays (FN)

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood . Immunohistology - Frozen: Minimum Dilution: 1/200; Maximum Dilution: 1/2000; Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections. . Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100

Immunogen: Human PHA blasts. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line.

Histology Positive Control Tissue: Human lymphatic tissue. Buffer Solution: Phosphate buffered saline. Target Species: Human. Preservative Stabilizer: None Represent.

10835167

NP_000607.1

NM_000616.4

Adaptive Immune System Pathway (366160); Alpha-defensins Pathway (530758); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Arf1 Pathway (137927); Binding And Entry Of HIV Virion Pathway (105986); C-MYB Transcription Factor Network Pathway (138073); CXCR4-mediated Signaling Events Pathway (137910); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480)

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120. Protein type: Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 12p13.31. Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; plasma membrane; integral to membrane; lipid raft; external side of plasma membrane. Molecular Function: protein binding; transmembrane receptor activity; protein homodimerization activity; enzyme binding; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding. Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine production; T cell selection; positive regulation of calcium-mediated signaling; induction by virus of cell-cell fusion in host; defense response to Gram-negative bacterium; signal transduction; enzyme linked receptor protein signaling pathway; T cell receptor signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; protein palmitoleylation; T cell costimulation; innate immune response; entry into host cell; immune response; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation; regulation of T cell activation. Disease: Okt4 Epitope Deficiency

0.5 mg

550 USD