MOUSE ANTI HUMAN B CELLS:FITC | MyBioSource MBS211102 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN B CELLS:FITC

catalog :

MBS211102

quantity :

100 Tests

price :

625 USD

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

FMC7

reactivity :

human

application :

flow cytometry, FACS

product information

catalog number :

MBS211102

products type :

Antibody

products full name :

MOUSE ANTI HUMAN B CELLS:FITC

products short name :

B CELLS

other names :

B-lymphocyte antigen CD20; B-lymphocyte antigen CD20; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; membrane-spanning 4-domains, subfamily A, member 1; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD_antigen: CD20

other gene names :

MS4A1; MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16; CD20

uniprot entry name :

CD20_HUMAN

clonality :

Monoclonal

isotype :

IgM

clone :

FMC7

host :

Mouse

sequence length :

130

specificity :

This item recognises a glycoprotein antigen of 105kD expressed by B lymphocytes. The FMC7 antigen is expressed by peripheral B lymphocytes, and has been used widely in the study of B cell malignancy. FMC7 has been used extensively to differentiate various types of B cell malignancy. B-CLL is generally considered to be negative for FMC7 expression, but strong staining is seen in many other types of B cell lymphoma, including prolymphocytic leukaemia and hairy cell leukaemia.The nature of the FMC7 antigen has remained poorly defined following its first description in 1981. The expression pattern closely corresponds to that seen with CD22, but studies have shown that FMC7 does not recognise the CD22 molecule itself. A recent publication by Serke et al. has shown that FMC7 recognises a conformational epitope on the CD20 molecule, most likely a multimeric complex of CD20.

form :

FITC. Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Flow cytometry (FC/FACS)

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Flow Cytometry: Maximum Dilution: Neat

other info1 :

Perservative Stabilisers: 0.1% Sodium Azide (NaN3). 0.2% Bovine Serum Albumin . Preparation: Purified IgM prepared by gel filtration

other info2 :

Immunogen: HRIK cells - Human B-Lymphoblastoid line. Buffer Solution: TRIS buffered glycine. Target Species: Human

products description :

MBS211102 recognises a glycoprotein antigen of 105kD expressed by B lymphocytes. The FMC7 antigen is expressed by peripheral B lymphocytes, and has been used widely in the study of B cell malignancy. FMC7 has been used extensively to differentiate various types of B cell malignancy. B-CLL is generally considered to be negative for FMC7 expression, but strong staining is seen in many other types of B cell lymphoma, including prolymphocytic leukaemia and hairy cell leukaemia. The nature of the FMC7 antigen has remained poorly defined following its first description in 1981. The expression pattern closely corresponds to that seen with CD22, but studies have shown that FMC7 does not recognise the CD22 molecule itself. A recent publication by Serke et al. has shown that FMC7 recognises a conformational epitope on the CD20 molecule, most likely a multimeric complex of CD20.

ncbi gi num :

23110987

ncbi acc num :

NP_068769.2

ncbi gb acc num :

NM_021950.3

uniprot acc num :

P11836

ncbi mol weight :

14,581 Da

ncbi pathways :

Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489)

ncbi summary :

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]

uniprot summary :

MS4A1: This protein may be involved in the regulation of B-cell activation and proliferation. Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the MS4A family. Protein type: Membrane protein, multi-pass; Cell surface; Membrane protein, integral. Chromosomal Location of Human Ortholog: 11q12. Cellular Component: extracellular space; integral to plasma membrane; external side of plasma membrane. Molecular Function: epidermal growth factor receptor binding. Biological Process: B cell proliferation; humoral immune response. Disease: Immunodeficiency, Common Variable, 5

size1 :

100 Tests

price1 :

625 USD