RAT ANTI HUMAN CD49f:FITC | MyBioSource MBS211073 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RAT ANTI HUMAN CD49f:FITC

catalog :

MBS211073

quantity :

0.1 mg

price :

370 USD

clonality :

monoclonal

host :

rat

conjugate :

FITC

clone name :

[NKI-GoH3]

reactivity :

human, mouse, dog, cow

application :

flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Staining of human peripheral blood lymphocytes with Rat anti Human CD49f:Biotin (MBS210508)

image 2 :

Staining of human peripheral blood platelets with Rat anti Human CD49f:Biotin (MBS210508)

product information

catalog number :

MBS211073

products type :

Antibody

products full name :

RAT ANTI HUMAN CD49f:FITC

products short name :

[CD49f]

products name syn :

[INTEGRIN ALPHA 6 CHAIN, VLA-6]

other names :

[integrin alpha-6 isoform b; Integrin alpha-6; integrin alpha-6; CD49 antigen-like family member F; integrin alpha6B; integrin, alpha 6; CD49 antigen-like family member F; VLA-6; CD_antigen: CD49fCleaved into the following 3 chains:Integrin alpha-6 heavy chain; Integrin alpha-6 light chain; Processed integrin alpha-6; Alpha6p]

products gene name :

[CD49f]

other gene names :

[ITGA6; ITGA6; CD49f; VLA-6; ITGA6B; Alpha6p]

uniprot entry name :

ITA6_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

[NKI-GoH3]

host :

Rat

reactivity :

Reacts with Mouse, Dog, Pig, Cynomolgus monkey, Sheep. N.B. Antibody reactivity and working conditions may vary between species.

sequence length :

1,097

form :

FITC. Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

concentration :

IgG concentration 0.1 mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Flow cytometry (FC/FACS)

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Maximum Dilution: Neat

image1 heading :

Testing Data

image2 heading :

Testing Data

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. 1% Bovine Serum Albumin. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

other info2 :

Buffer Solution: Phosphate buffered saline. Target Species: Human. Immunogen: BALB/c mouse mammary tumor cells. Fusion Partners: Spleen cells from immunised Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line.

products description :

Rat anti Human CD49f antibody, clone NKI-GoH3 recognizes CD49F (VLA-6 alpha chain), a 120kD cell surface glycoprotein that can form distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells. Clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts.VLA-6 is an important mediator of cell binding to laminin. Studies suggest that clone NKI-GoH3 specifically blocks the binding of cells to the E8 fragment of laminin (Sonnenberg et al. 1998).

ncbi gi num :

119395742

ncbi acc num :

NP_000201.2

ncbi gb acc num :

NM_000210.2

uniprot acc num :

P23229

ncbi mol weight :

122,358 Da

ncbi pathways :

Alpha6-Beta4 Integrin Signaling Pathway (198807); Arf6 Trafficking Events Pathway (137954); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Basigin Interactions Pathway (106065); CXCR4-mediated Signaling Events Pathway (137910); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480)

ncbi summary :

The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

ITGA6: Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with HPS5. Interacts with RAB21. Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). Belongs to the integrin alpha chain family. 8 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis; Cell adhesion. Chromosomal Location of Human Ortholog: 2q31.1. Cellular Component: focal adhesion; cell-cell adherens junction; cell surface; hemidesmosome; plasma membrane; basal plasma membrane; basement membrane; filopodium; external side of plasma membrane. Molecular Function: integrin binding; protein binding; metal ion binding; laminin binding. Biological Process: integrin-mediated signaling pathway; skin development; extracellular matrix organization and biogenesis; filopodium formation; positive regulation of apoptosis; cell-matrix adhesion; cell-substrate adhesion; cell-cell adhesion; hemidesmosome assembly; gut development; cell-substrate junction assembly; brown fat cell differentiation; cellular response to extracellular stimulus; positive regulation of cell-cell adhesion; cell adhesion mediated by integrin; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; leukocyte migration; positive regulation of phosphorylation. Disease: Epidermolysis Bullosa Junctionalis With Pyloric Atresia

size1 :

0.1 mg

price1 :

370 USD

more info or order :

MyBioSource product webpage