catalog number :
MBS211025
products full name :
MOUSE ANTI RAT MHC CLASS II RT1B:FITC
products short name :
MHC CLASS II RT1B
form :
FITC. Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
concentration :
IgG concentration 0.1 mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Flow cytometry (FC/FACS)
app notes :
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide. 1% Bovine Serum Albumin. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
other info2 :
Immunogen: Rat thymocyte membrane glycoproteins. Fusion Partners: Spleen cells from immunised BALB/C mice were fused with cells of the mouse NS1 myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Rat
products description :
Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 recognizes a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Rat MHC Class II RT1B antibody, clone OX-6 does not react with the rat BDIX strain due to a defect in RT1B expression (Male et al. 1987). The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region. Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region (McMaster and Williams 1979 and Male et al. 1987). This product is routinely tested in flow cytometry on rat splenocytes.