MOUSE ANTI HUMAN GLUCOCORTICOID RECEPTOR:FITC | MyBioSource MBS210980 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

MOUSE ANTI HUMAN GLUCOCORTICOID RECEPTOR:FITC

catalog :

MBS210980

quantity :

0.1 mg

price :

610 USD

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

5.00E+04

reactivity :

human

application :

flow cytometry, FACS

product information

catalog number :

MBS210980

products type :

Antibody

products full name :

MOUSE ANTI HUMAN GLUCOCORTICOID RECEPTOR:FITC

products short name :

GLUCOCORTICOID RECEPTOR

other names :

glucocorticoid receptor isoform alpha; Glucocorticoid receptor; glucocorticoid receptor; glucocorticoid nuclear receptor variant 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); Nuclear receptor subfamily 3 group C member 1

other gene names :

NR3C1; NR3C1; GR; GCR; GRL; GCCR; GCRST; GRL; GR

uniprot entry name :

GCR_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

5.00E+04

host :

Mouse

sequence length :

777

form :

FITC. Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

concentration :

IgG concentration 0.1 mg/ml

storage stability :

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

tested application :

Flow cytometry (FC/FACS)*

app notes :

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.

other info1 :

Perservative Stabilisers: 0.09% Sodium Azide. 1% Bovine Serum Albumin. Preparation: Purified IgG prepared by ion exchange chromatography from tissue culture supernatant

other info2 :

Immunogen: 26 amino acid peptide corresponding to residues 150-176 on human GCR linked to thyroglobulin. Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the mouse Sp-2/0 Ag14 myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Human

products description :

Mouse anti Human Glucocorticoid Receptor antibody, clone 5E4 recognizes the human glucocorticoid receptor, also known as Nuclear receptor subfamily 3 group C member 1 (NR3C1), a 777 amino acid receptor bearing 3 distinct functional domains, an N-terminal modulating domain, a DNA binding domain and a C-terminal steroid binding domain. The human glucocorticoid receptor is located either in the cytoplasm of cells, prior to ligand binding associated with certain heat shock proteins (Kino et al. 2009), or the nucleus following binding to ligand (Werb et al. 1978). Mutations in the glucocorticoid receptor gene can lead to familial glucocorticoid resistance, characterized by elevated plasma cortisol levels (Malchoff et al. 1993). Mouse anti human glucocorticoid receptor antibody, clone 5E4 has been demonstrated to cross-react with the murine glucocorticoid receptor (Bergquist et al. 2014)

ncbi gi num :

4504133

ncbi acc num :

NP_000167.1

ncbi gb acc num :

NM_000176.2

uniprot acc num :

P04150

ncbi mol weight :

82,904 Da

ncbi pathways :

Adipogenesis Pathway 198832!!BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway 477138!!Circadian Clock Pathway 187173!!FOXA2 And FOXA3 Transcription Factor Networks Pathway 137911!!Gene Expression Pathway 105937!!Generic Transcription Pathway 105938!!Glucocorticoid Receptor Regulatory Network Pathway 138014!!Neuroactive Ligand-receptor Interaction Pathway 83053!!Neuroactive Ligand-receptor Interaction Pathway 462!!Nuclear Receptor Transcription Pathway 105979

ncbi summary :

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]

size :

0.1 mg

price :

610 USD