catalog number :
MBS210925
products full name :
RAT ANTI MOUSE CD49d:FITC
products short name :
CD49d
other names :
integrin alpha-4; Integrin alpha-4; integrin alpha-4; CD49 antigen-like family member D; LPAM subunit alpha; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha; integrin alpha-IV; integrin alpha4B; lymphocyte Peyer patch adhesion molecules subunit alpha; integrin alpha 4; CD49 antigen-like family member D; Integrin alpha-IV; Lymphocyte Peyer patch adhesion molecules subunit alpha; LPAM subunit alpha; VLA-4 subunit alpha; CD_antigen: CD49d
products gene name :
CD49d
other gene names :
Itga4; Itga4; CD49D; Itga4B; LPAM subunit alpha
uniprot entry name :
ITA4_MOUSE
form :
FITC. Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
concentration :
IgG concentration 0.1 mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Flow cytometry (FC/FACS)
app notes :
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR. Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/2
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide. 1% Bovine Serum Albumin. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
other info2 :
Buffer Solution: Phosphate buffered saline. Target Species: Mouse
products description :
Rat anti Mouse CD49d monoclonal antibody, clone 9C10 recognizes murine alpha 4 integrin (CD49d) also known as VLA-4 subunit alpha, CD49d is a ~150kD single pass type I transmembrane glycoprotein that can associate with either beta 1 integrin (CD29) or beta 7 integrin to form heterodimers CD49d/CD29 (VLA-4) and alpha4/beta7 (LPAM-1) respectively. CD49d is expressed on most lymphocytes, granulocytes, monocytes and thymocytes. The primary ligands for CD49d are CD106 (VCAM-1), fibronectin and MAdCAM-1. Clone 9C10 (MFR4.B) is reported to significantly inhibit binding of CD49d to CD106 but only when used in combination with clone R1-2 (Kinashi, T. et al.)
ncbi acc num :
NP_034706.3
ncbi gb acc num :
NM_010576.3
ncbi mol weight :
115,696 Da
ncbi pathways :
Adaptive Immune System Pathway 1110669!!Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway 117303!!Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway 116129!!Cell Adhesion Molecules (CAMs) Pathway 83266!!Cell Adhesion Molecules (CAMs) Pathway 480!!Cell Surface Interactions At The Vascular Wall Pathway 1110059!!Dilated Cardiomyopathy Pathway 121495!!Dilated Cardiomyopathy Pathway 121285!!ECM-receptor Interaction Pathway 83265!!ECM-receptor Interaction Pathway 479