catalog number :
MBS210809
products full name :
RAT ANTI MOUSE CD68:FITC
products short name :
CD68
other names :
Macrosialin; macrosialin; CD68 antigen; CD_antigen: CD68
products gene name :
CD68
other gene names :
Cd68; Cd68; Lamp4; gp110; Scard1
uniprot entry name :
CD68_MOUSE
form :
FITC. Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
concentration :
IgG concentration 0.1mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Flow cytometry (FC/FACS)*
app notes :
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide. 1% Bovine Serum Albumin. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
other info2 :
Immunogen: Purified Concanavalin A acceptor glycoprotein from P815 cell line. Buffer Solution: Phosphate buffered saline. Target Species: Mouse
ncbi acc num :
NP_033983.1
ncbi gb acc num :
NM_009853.1
ncbi mol weight :
33,844 Da
ncbi pathways :
Lysosome Pathway (99272); Lysosome Pathway (96865); Macrophage Markers Pathway (672439)
uniprot summary :
CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell surface; Membrane protein, integral. Cellular Component: membrane; lysosome; plasma membrane; integral to membrane; endosome