antibody

MOUSE ANTI HUMAN HLA B27:FITC

MBS210626

100 Tests

600 USD

monoclonal

mouse

FITC

HLA-ABC-m3

flow cytometry, FACS

Antibody

MOUSE ANTI HUMAN HLA B27:FITC

HLA B27

HLA class I histocompatibility antigen, B-27 alpha chain; HLA class I histocompatibility antigen, B-27 alpha chain; major histocompatibility complex, class I, B; HLA class I histocompatibility antigen, B alpha chain; MHC Class I HLA heavy chain; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC class I antigen GN00104; MHC class I antigen HLA-B heavy chain; MHC class I antigen SHCHA; leukocyte antigen class I-B; lymphocyte antigen; major histocompatibility complex, class I, B; MHC class I antigen B*27

HLA B27

HLA-B; HLA-B; AS; HLAB; SPDA1; HLAB

1B27_HUMAN

Monoclonal

IgG2a

HLA-ABC-m3

Mouse

362

FITC. Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Approximate Protein Concentration: 50 ug/ml

Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Flow cytometry (FC/FACS)

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul of whole blood. Flow Cytometry: Maximum Dilution: Neat

Perservative Stabilisers: 0.1% Sodium Azide (NaN3). 0.2% Bovine Serum Albumin . Preparation: Purified IgG prepared by ion exchange chromatography

Immunogen: Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A. Buffer Solution: Phosphate buffered saline. Target Species: Human

Mouse anti Human HLA B27 antibody, clone HLA-ABC-m3 recognizes the HLA-B27 alloantigen. This antibody reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin. Affinity studies by Scatchard analysis showed that this antibody has a higher affinity for HLA-B27 (9.7 x 108M-1) than for HLA B7 (9.5 x 107 M-1). Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative

34305677

P03989.2

P03989

40,428 Da

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (477122); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533)

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]

HLAB iso7: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family. Protein type: Membrane protein, integral; Immunoglobulin superfamily. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; membrane; early endosome membrane; integral to plasma membrane; endoplasmic reticulum; plasma membrane; integral to membrane; MHC class I protein complex. Molecular Function: peptide antigen binding; chaperone binding; TAP binding; receptor binding. Biological Process: regulation of immune response; regulation of interleukin-12 production; viral reproduction; detection of bacterium; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; defense response; protection from natural killer cell mediated cytotoxicity; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; regulation of T cell anergy; regulation of interleukin-6 production; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; immune response

100 Tests

600 USD