catalog number :
MBS210518
products full name :
MOUSE ANTI RAT CD54:Biotin
products short name :
CD54
other names :
intercellular adhesion molecule 1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1; ICAM-1; intercellular adhesion molecule 1; CD_antigen: CD54
products gene name :
CD54
other gene names :
Icam1; Icam1; CD54; ICAM; Icam-1; ICAM-1
uniprot entry name :
ICAM1_RAT
form :
Biotin. Purified IgG conjugated to Biotin - liquid
concentration :
IgG concentration 0.1 mg/ml
storage stability :
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
tested application :
Flow cytometry (FC/FACS)
app notes :
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/5
other info1 :
Perservative Stabilisers: 0.09% Sodium Azide. 1% Bovine Serum Albumin. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
other info2 :
Immunogen: Rat Ax cells (a HEV derived cell line). Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Rat
products description :
Mouse anti Rat CD54 antibody, clone 1A29 recognizes the rat CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1), a 90kD adhesion molecule belonging to the immunoglobulin superfamily. CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system. Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling (Etienne, S. et al.). Functionally 1A29 inhibits homotypic aggregation of PHA blasts.
ncbi acc num :
NP_037099.1
ncbi gb acc num :
NM_012967.1
ncbi mol weight :
60,142 Da
ncbi pathways :
Adaptive Immune System Pathway 1121508!!African Trypanosomiasis Pathway 194377!!African Trypanosomiasis Pathway 194323!!Cell Adhesion Molecules (CAMs) Pathway 83461!!Cell Adhesion Molecules (CAMs) Pathway 480!!Epstein-Barr Virus Infection Pathway 585584!!Epstein-Barr Virus Infection Pathway 587115!!Extracellular Matrix Organization Pathway 1121481!!HTLV-I Infection Pathway 373894!!HTLV-I Infection Pathway 373889
ncbi summary :
cell adhesion molecule; ligand for leukocyte adhesion molecule LFA-1 [RGD, Feb 2006]