antibody

RAT ANTI HUMAN CD49f:Biotin

MBS210508

0.1 mg

390 USD

monoclonal

rat

biotin

NKI-GoH3

flow cytometry, FACS

Antibody

RAT ANTI HUMAN CD49f:Biotin

CD49f

integrin alpha-6 isoform b; Integrin alpha-6; integrin alpha-6; CD49 antigen-like family member F; integrin alpha6B; integrin, alpha 6; CD49 antigen-like family member F; VLA-6; CD_antigen: CD49fCleaved into the following 3 chains:Integrin alpha-6 heavy chain; Integrin alpha-6 light chain; Processed integrin alpha-6; Alpha6p

CD49f

ITGA6; ITGA6; CD49f; VLA-6; ITGA6B; Alpha6p

ITA6_HUMAN

Monoclonal

IgG2a

NKI-GoH3

Rat

1073

Biotin. Purified IgG conjugated to Biotin - liquid

IgG concentration 0.1 mg/ml

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Flow cytometry (FC/FACS)

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 platelets in 100ul. Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/5

Perservative Stabilisers: 0.09% Sodium Azide. 1% Bovine Serum Albumin. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution: Phosphate buffered saline. Target Species: Human

Rat anti Human CD49f antibody, clone NKI-GoH3 recognizes CD49F (VLA-6 alpha chain), a 120kD cell surface glycoprotein that can form distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells. Clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts.VLA-6 is an important mediator of cell binding to laminin. Studies suggest that clone NKI-GoH3 specifically blocks the binding of cells to the E8 fragment of laminin (Sonnenberg et al. 1998).

119395742

NP_000201.2

NM_000210.2

P23229

122,358 Da

Alpha6-Beta4 Integrin Signaling Pathway (198807); Arf6 Trafficking Events Pathway (137954); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Basigin Interactions Pathway (106065); CXCR4-mediated Signaling Events Pathway (137910); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480)

The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

ITGA6: Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with HPS5. Interacts with RAB21. Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). Belongs to the integrin alpha chain family. 8 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cell adhesion; Receptor, misc.; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 2q31.1. Cellular Component: cell surface; focal adhesion; cell-cell adherens junction; hemidesmosome; basal plasma membrane; plasma membrane; basement membrane; filopodium; external side of plasma membrane. Molecular Function: integrin binding; protein binding; metal ion binding; laminin binding. Biological Process: skin development; integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; filopodium formation; positive regulation of apoptosis; cell-matrix adhesion; cell-substrate adhesion; cell-cell adhesion; hemidesmosome assembly; gut development; cell-substrate junction assembly; positive regulation of cell-cell adhesion; cellular response to extracellular stimulus; brown fat cell differentiation; cell adhesion mediated by integrin; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; leukocyte migration; positive regulation of phosphorylation. Disease: Epidermolysis Bullosa Junctionalis With Pyloric Atresia

0.1 mg

390 USD