catalog number :
MBS2104718
products type :
ELISA Kit
products full name :
Canine Superoxide Dismutases (SOD) ELISA Kit
products short name :
[Superoxide Dismutases (SOD)]
products gene name :
[SOD]
specificity :
This assay has high sensitivity and excellent specificity for detection of SOCS3. No significant cross-reactivity or interference between SOCS3 and analogues was observed.
storage stability :
For unopened kit, all the reagents should be kept according to the label on each vial. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C while others should be at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Human Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.6-1,000pg/mL. Sensitivity: 6.1pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SOCS3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SOCS3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SOCS3 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to SOCS3. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to SOCS3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SOCS3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SOCS3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
size5 :
10x96-Strip-Wells