catalog number :
MBS2104692
products type :
ELISA Kit
products full name :
Chicken LuteinizingHormone (LH) ELISA Kit
products short name :
[LuteinizingHormone (LH)]
products gene name :
[LH]
specificity :
This assay has high sensitivity and excellent specificity for detection of LTB4. No significant cross-reactivity or interference between LTB4 and analogues was observed.
storage stability :
For unopened kit, all the reagents should be kept according to the label on each vial. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C while others should be at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 246.9-20,000pg/mL. Sensitivity: 90.1pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LTB4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LTB4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of LTB4 in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to LTB4 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled LTB4 and unlabeled LTB4 (Standards or samples) with the pre-coated antibody specific to LTB4. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of LTB4 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of LTB4 in the sample.
size5 :
10x96-Strip-Wells