antibody

MOUSE ANTI HUMAN CD27:Preservative Free

MBS210268

1 mg

935 USD

monoclonal

mouse

nonconjugated

LT27

immunoprecipitation, flow cytometry, FACS

Antibody

MOUSE ANTI HUMAN CD27:Preservative Free

CD27

CD27 antigen; CD27 antigen; CD27 antigen; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7; CD27 molecule; CD27L receptor; T-cell activation antigen CD27; T14; Tumor necrosis factor receptor superfamily member 7; CD_antigen: CD27

CD27

CD27; CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2; TNFRSF7

CD27_HUMAN

Monoclonal

IgG2a

LT27

Mouse

260

Preservative Free. Purified IgG - liquid

IgG concentration 1.0 mg/ml

Store at -20 degree C only. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.

Flow cytometry (FC/FACS), Immunoprecipitation (IP)

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Maximum Dilution: 1/100

Preparation: Purified IgG prepared by ion exchange chromatography

Immunogen: Normal human blood lymphocytes. Fusion Partners: Spleen cells from immunised mice were fused with cells of the P3. X 63-Ag8-653 mouse myeloma cell line. Buffer Solution: Phosphate buffered saline. Target Species: Human

Mouse anti human CD27, clone LT27, recognizes the human T-cell activation antigen, CD27. This antigen is expressed on activated T lymphocytes and belongs to a family of proteins associated with the nerve growth factor receptor. CD27 is expressed by thymocytes and peripheral T cells and also by EBV transformed B cell lines. Mouse anti human CD27, clone LT27 precipitates a 55kDa protein under reducing conditions or a 120kDa protein under non-reducing conditions.

4507587

NP_001233.1

NM_001242.4

P26842

29,137 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460)

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]

CD27: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. Protein type: Membrane protein, integral; Receptor, cytokine; Apoptosis. Chromosomal Location of Human Ortholog: 12p13. Cellular Component: integral to plasma membrane; extracellular region; external side of plasma membrane. Molecular Function: protein binding; transmembrane receptor activity; caspase inhibitor activity. Biological Process: release of cytoplasmic sequestered NF-kappaB; response to ethanol; cell surface receptor linked signal transduction; positive regulation of JNK cascade; positive regulation of B cell differentiation; negative regulation of caspase activity; immunoglobulin mediated immune response; negative regulation of apoptosis. Disease: Lymphoproliferative Syndrome 2

1 mg

935 USD