catalog number :
MBS210038
products full name :
MOUSE ANTI HUMAN CYTOKERATIN
products short name :
CYTOKERATIN
other names :
cytokeratin; Keratin, type II cytoskeletal 8; keratin, type II cytoskeletal 8; cytokeratin 8; cytokeratin-8; type-II keratin Kb8; keratin 8, type II; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
other gene names :
KRT8; KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2; CYK8; CK-8; K8
uniprot entry name :
K2C8_HUMAN
reactivity :
Bovine, Chicken, Horse, Rat, Xenopus
specificity :
Clone Lu-5 recognizes an intracytoplasmic, formaldehyde-resistant epitope on the surface of cytokeratin filaments.
form :
Concentrated Tissue Culture Supernatant - lyophilised
storage stability :
Prior to reconstitution store at 4 degree C. After reconstitution store at 4 degree C or -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of reconstitution.
tested application :
Immunohistology Frozen, Immunohistology Paraffin*
app notes :
Immunohistology - Frozen: Minimum Dilution: 1/500; Maximum Dilution: 1/1000;. Immunohistology - Paraffin: Minimum Dilution: 1/200; Application Note: This product requires protein digestion pre-treatment of paraffin sections. Proteinase K is recommended for this purpose.
other info1 :
Reconstitution: Reconstitute with 0.5ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. Perservative Stabilisers: 0.05% Sodium Azide (NaN3)
other info2 :
Immunogen: Human Lung Cancer Cell Line. Histology Positive Control Tissue: Human Tonsil. Buffer Solution: Phosphate buffered saline. Target Species: Human
products description :
Clone Lu-5 recognizes an intracytoplasmic, formaldehyde-resistant epitope on the surface of cytokeratin filaments.
ncbi acc num :
CAA67203.1
ncbi mol weight :
56,608 Da
ncbi pathways :
EGFR1 Signaling Pathway (198782); Signaling Mediated By P38-alpha And P38-beta Pathway (138012)
ncbi summary :
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
uniprot summary :
K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 12q13. Cellular Component: nucleoplasm; dystrophin-associated glycoprotein complex; intermediate filament cytoskeleton; costamere; nuclear matrix; keratin filament; cytoplasm; intermediate filament; intercellular junction; nucleus; Z disc; sarcolemma. Molecular Function: protein binding; protein complex binding; structural molecule activity. Biological Process: tumor necrosis factor-mediated signaling pathway; viral reproduction; response to other organism; sarcomere organization; response to hydrostatic pressure. Disease: Cirrhosis, Familial