catalog number :
MBS210027
products full name :
RAT ANTI MOUSE CD8 ALPHA:APC
products short name :
CD8 ALPHA
other names :
T-cell surface glycoprotein CD8 alpha chain isoform 1; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell surface glycoprotein Lyt-2; CD8 antigen, alpha chain; T-cell surface glycoprotein Lyt-2; CD_antigen: CD8a
products gene name :
CD8 ALPHA
other gene names :
Cd8a; Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331; Lyt-2; Lyt2
uniprot entry name :
CD8A_MOUSE
form :
APC. Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
storage stability :
Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of reconstitution.
tested application :
Flow cytometry (FC/FACS)
app notes :
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR. Flow Cytometry: Maximum Dilution: Neat
other info1 :
Reconstitution: Reconstitute with 1 ml distilled water. Perservative Stabilisers: 0.09% Sodium Azide. 1% Bovine Serum Albumin. 5% Sucrose
other info2 :
Buffer Solution: Phosphate buffered saline. Target Species: Mouse
ncbi acc num :
NP_001074579.1
ncbi gb acc num :
NM_001081110.2
ncbi mol weight :
27,456 Da
ncbi pathways :
Adaptive Immune System Pathway (1110669); Antigen Processing And Presentation Pathway (83271); Antigen Processing And Presentation Pathway (485); Cell Adhesion Molecules (CAMs) Pathway (83266); Cell Adhesion Molecules (CAMs) Pathway (480); Hematopoietic Cell Lineage Pathway (83275); Hematopoietic Cell Lineage Pathway (489); Immune System Pathway (1110668); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (1110695); Primary Immunodeficiency Pathway (83318)
uniprot summary :
CD8A: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane. Molecular Function: protein binding; protein homodimerization activity; protein kinase binding. Biological Process: cell surface receptor linked signal transduction; T cell activation; immune system process; T cell mediated immunity; positive regulation of calcium-mediated signaling; cytotoxic T cell differentiation; defense response to virus