ELISA/assay

Pig Syndecan 1 (SDC1) ELISA Kit

MBS2097984

24-Strip-Wells

290 USD

ELISA Kit

Pig Syndecan 1 (SDC1) ELISA Kit

[Syndecan 1 (SDC1)]

[CD138; SDC; SYND1; Syndecan Proteoglycan 1]

[syndecan-1; Syndecan-1; syndecan-1; syndecan 1; CD_antigen: CD138]

[SDC1]

[SDC1; SDC1; SDC; CD138; SYND1; syndecan; SDC; SYND1]

Porcine

310

This assay has high sensitivity and excellent specificity for detection of SCUBE1. No significant cross-reactivity or interference between SCUBE1 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Rat Tissue Homogenates, Cell Lysates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 78-5,000pg/mL. Sensitivity: 32pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SCUBE1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SCUBE1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Tumor immunity; Infection immunity; Immunodeficiency

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SCUBE1 in rat tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to SCUBE1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to SCUBE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SCUBE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SCUBE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

55749480

NP_001006947.1

NM_001006946.1

P18827

32,462 Da

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (1269981); Arf6 Trafficking Events Pathway (137954); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (1269373); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (1269984); Defective B3GALT6 Causes EDSP2 And SEMDJL1 Pathway (1309217); Defective B3GAT3 Causes JDSSDHD Pathway (1269015); Defective B4GALT7 Causes EDS, Progeroid Type Pathway (1269014); Defective EXT1 Causes Exostoses 1, TRPS2 And CHDS Pathway (1269021)

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]

syndecan-1: Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Belongs to the syndecan proteoglycan family. Protein type: Extracellular matrix; Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 2p24.1. Cellular Component: cell surface; external side of plasma membrane; extracellular exosome; Golgi lumen; integral component of plasma membrane; lysosomal lumen; plasma membrane; protein complex. Molecular Function: identical protein binding; protein binding; protein C-terminus binding. Biological Process: cell migration; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; inflammatory response; leukocyte migration; myoblast development; odontogenesis; response to calcium ion; response to cAMP; response to glucocorticoid stimulus; response to hydrogen peroxide; response to toxin; retinoid metabolic process; Sertoli cell development; striated muscle cell development; ureteric bud development; Wnt receptor signaling pathway through beta-catenin; wound healing

24-Strip-Wells

290 USD

48-Strip-Wells

550

96-Strip-Wells

750

5x96-Strip-Wells

3040

10x96-Strip-Wells

5485