protein

Recombinant Atrial Natriuretic Peptide (ANP)

MBS2097708

0.01 mg

130 USD

Recombinant Protein

Recombinant Atrial Natriuretic Peptide (ANP)

[Atrial Natriuretic Peptide (ANP)]

[ANH; ANF; CDD; Atrial Natriuretic Factor; Atrial Natriuretic Hormone; Atriopeptin; Cardionatrine; Cardiodilatin]

[natriuretic peptides A preproprotein; Natriuretic peptides A; natriuretic peptides A; natriuretic peptide A; CDD-ANF; Cardiodilatin; CDD]

[ANP]

[NPPA; NPPA; ANF; ANP; CDD; CDP; PND; ATFB6; ATRST2; CDD-ANF; ANP; PND; CDD; CDP; ANF; ANP]

E Coli

> 95%

Original concentration: 200 ug/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control; Immunogen; SDS%PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

SDS-Page

Organism Species: Homo sapiens. Source: Prokaryotic expression. Residues: Lys41~Tyr151. Tags: N-terminal His and GST Tag. Subcellular Location: Secreted. Traits: Freeze-dried powder. Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

Predicted isoelectric point: 5.9. Predicted Molecular Mass: 42.0kDa. Accurate Molecular Mass: 48kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1.Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Endocrinology; Cardiovascular biology; Hormone metabolism

23510319

NP_006163.1

NM_006172.3

P01160

Amyloids Pathway (366238); Disease Pathway (530764); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); HIF-1 Signaling Pathway (695200); MicroRNAs In Cardiomyocyte Hypertrophy Pathway (198784); YAP1- And WWTR1 (TAZ)-stimulated Gene Expression Pathway (576276)

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1. [provided by RefSeq, Oct 2015]

NPPA: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Defects in NPPA are the cause of familial atrial fibrillation type 6 (ATFB6). Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity, progressive deterioration of atrial electromechanical function and ineffective pumping of blood into the ventricles. It can be associated with palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the natriuretic peptide family. Protein type: Hormone; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1p36.22. Cellular Component: extracellular matrix; extracellular region; extracellular space; mast cell granule; nucleus; perinuclear region of cytoplasm; protein complex. Molecular Function: neuropeptide hormone activity; peptide hormone receptor binding; protein binding; receptor binding. Biological Process: cardiac muscle hypertrophy in response to stress; cellular protein metabolic process; cGMP biosynthetic process; female pregnancy; negative regulation of cell growth; negative regulation of systemic arterial blood pressure; neuropeptide signaling pathway; positive regulation of heart rate; protein folding; receptor guanylyl cyclase signaling pathway; regulation of blood pressure; regulation of blood vessel size; response to hypoxia; response to insulin stimulus; transcription initiation from RNA polymerase II promoter. Disease: Atrial Fibrillation, Familial, 6; Atrial Standstill 2

0.01 mg

130 USD

0.05 mg

225

0.1 mg

340

0.2 mg

415

0.5 mg

800

1 mg

1180